Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration.

Autor: Esteca MV; Laboratory of Cell and Tissue Biology, School of Applied Sciences, University of Campinas, 13484-350 Limeira, Brazil., Severino MB; Laboratory of Cell and Tissue Biology, School of Applied Sciences, University of Campinas, 13484-350 Limeira, Brazil., Silvestre JG; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, 05508-900 São Paulo, Brazil., Palmeira Dos Santos G; Laboratory of Cell and Tissue Biology, School of Applied Sciences, University of Campinas, 13484-350 Limeira, Brazil., Tamborlin L; Laboratory of Biotechnology, School of Applied Sciences, University of Campinas, 13484-350 Limeira, Brazil.; Institute of Biosciences, São Paulo State University, 13506-900 Rio Claro, Brazil., Luchessi AD; Laboratory of Biotechnology, School of Applied Sciences, University of Campinas, 13484-350 Limeira, Brazil.; Institute of Biosciences, São Paulo State University, 13506-900 Rio Claro, Brazil., Moriscot AS; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, 05508-900 São Paulo, Brazil., Gustafsson ÅB; Skaggs School of Pharmacy and Pharmacological Sciences, University of California San Diego, La Jolla, CA 92093-0021, USA., Baptista IL; Laboratory of Cell and Tissue Biology, School of Applied Sciences, University of Campinas, 13484-350 Limeira, Brazil.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2020 Oct 28; Vol. 21 (21). Date of Electronic Publication: 2020 Oct 28.
DOI: 10.3390/ijms21218007
Abstrakt: The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin -/- ) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin -/- mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin -/- mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.
Databáze: MEDLINE
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