Mass Spectrometry-Based Metabolic Fingerprinting Contributes to Unveil the Role of RSUME in Renal Cell Carcinoma Cell Metabolism.

Autor: Martinefski MR; Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina.; Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, C1113AAD Ciudad de Buenos Aires, Argentina., Elguero B; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina., Knott ME; Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina., Gonilski D; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina., Tedesco L; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina., Gurevich Messina JM; Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina., Pollak C; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina., Arzt E; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Ciudad de Buenos Aires, Argentina., Monge ME; Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2021 Jan 01; Vol. 20 (1), pp. 786-803. Date of Electronic Publication: 2020 Oct 30.
DOI: 10.1021/acs.jproteome.0c00655
Abstrakt: Clear cell renal cell carcinoma (ccRCC) is a heterogeneous disease with 50-80% patients exhibiting mutations in the von Hippel-Lindau (VHL) gene. RSUME (RWD domain (termed after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases)-containing protein small ubiquitin-related modifier (SUMO) enhancer) acts as a negative regulator of VHL function in normoxia. A discovery-based metabolomics approach was developed by means of ultraperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (MS) for fingerprinting the endometabolome of a human ccRCC cell line 786-O and three other transformed cell systems ( n = 102) with different expressions of RSUME and VHL. Cross-validated orthogonal projection to latent structures discriminant analysis models were built on positive, negative, and a combination of positive- and negative-ion mode MS data sets. Discriminant feature panels selected by an iterative multivariate classification allowed differentiating cells with different expressions of RSUME and VHL. Fifteen identified discriminant metabolites with level 1, including glutathione, butyrylcarnitine, and acetylcarnitine, contributed to understand the role of RSUME in ccRCC. Altered pathways associated with the RSUME expression were validated by biological and bioinformatics analyses. Combined results showed that in the absence of VHL, RSUME is involved in the downregulation of the antioxidant defense system, whereas in the presence of VHL, it acts in rerouting energy-related pathways, negatively modulating the lipid utilization, and positively modulating the fatty acid synthesis, which may promote deposition in droplets.
Databáze: MEDLINE