An Adolescent Porcine Model of Voluntary Alcohol Consumption Exhibits Binge Drinking and Motor Deficits in a Two Bottle Choice Test.

Autor:	Shin SK; Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA, 30602, USA.; Department of Pharmaceutical and Biomedical Sciences, Interdisciplinary Toxicology Institute, University of Georgia, 250 W. Green Street, Athens, GA, 30602, USA.; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, 425 River Road, Athens, GA, 30602, USA., Sneed SE; Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA, 30602, USA.; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, 425 River Road, Athens, GA, 30602, USA., Nennig SE; Department of Physiology and Pharmacy, College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Drive, Athens, GA, 30602, USA.; Neuroscience Program, Biomedical and Health Sciences Institute, University of Georgia, 310 East Campus Road, Athens, GA, 30602, USA., Cheek SR; Department of Pharmaceutical and Biomedical Sciences, Interdisciplinary Toxicology Institute, University of Georgia, 250 W. Green Street, Athens, GA, 30602, USA., Kinder HA; Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA, 30602, USA.; Department of Pharmaceutical and Biomedical Sciences, Interdisciplinary Toxicology Institute, University of Georgia, 250 W. Green Street, Athens, GA, 30602, USA.; Neuroscience Program, Biomedical and Health Sciences Institute, University of Georgia, 310 East Campus Road, Athens, GA, 30602, USA., Solomon MG; Department of Physiology and Pharmacy, College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Drive, Athens, GA, 30602, USA., Schank JR; Department of Physiology and Pharmacy, College of Veterinary Medicine, University of Georgia, 501 D.W. Brooks Drive, Athens, GA, 30602, USA.; Neuroscience Program, Biomedical and Health Sciences Institute, University of Georgia, 310 East Campus Road, Athens, GA, 30602, USA., West FD; Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA, 30602, USA.; Department of Pharmaceutical and Biomedical Sciences, Interdisciplinary Toxicology Institute, University of Georgia, 250 W. Green Street, Athens, GA, 30602, USA.; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, 425 River Road, Athens, GA, 30602, USA.; Neuroscience Program, Biomedical and Health Sciences Institute, University of Georgia, 310 East Campus Road, Athens, GA, 30602, USA.
Jazyk:	angličtina
Zdroj:	Alcohol and alcoholism (Oxford, Oxfordshire) [Alcohol Alcohol] 2021 Apr 29; Vol. 56 (3), pp. 266-274.
DOI:	10.1093/alcalc/agaa105
Abstrakt:	Aims: Alcohol is the most commonly abused substance leading to significant economic and medical burdens. Pigs are an attractive model for studying alcohol abuse disorder due to the comparable alcohol metabolism and consumption behavior, which are in stark contrast to rodent models. This study investigates the usage of a porcine model for voluntary binge drinking (BD) and a detailed analysis of gait changes due to motor function deficits during alcohol intoxication. Methods: Adolescent pigs were trained to drink increasing concentration (0-8%) of alcohol mixed in a 0.2% saccharin solution for 1 h in a two bottle choice test for 2 weeks. The training period was followed by a 3-week alcohol testing period, where animals were given free access to 8% alcohol in 0.2% saccharin solution and 0.2% saccharin water solution. Blood alcohol levels were tested and gait analysis was performed pre-alcohol consumption, last day of training, and Day 5 of each testing period. Results: Pigs voluntarily consumed alcohol to intoxication at all timepoints with blood alcohol concentration (BAL) ≥80 mg/dl. Spatiotemporal gait parameters including velocity, cadence, cycle time, swing time, stance time, step time, and stride length were perturbed as a result of intoxication. The stratification of the gait data based on BAL revealed that the gait parameters were affected in a dose-dependent manner. Conclusion: This novel adolescent BD porcine model with inherent anatomical and physiological similarities to humans display similar consumption and intoxication behavior that is likely to yield results that are translatable to human patients. (© The Author(s) 2020. Medical Council on Alcohol and Oxford University Press. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu