OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation.

Autor: Ohnishi K; Graduate School of Natural Science, Konan University, Kobe, 658-8501, Japan.; Institute for Integrative Neurobiology, Konan University, Kobe, 658-8501, Japan., Saito S; Division of Cell Signaling, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8787, Japan.; Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan., Miura T; Institute for Integrative Neurobiology, Konan University, Kobe, 658-8501, Japan., Ohta A; Graduate School of Natural Science, Konan University, Kobe, 658-8501, Japan.; Faculty of Science and Engineering, Konan University, Kobe, 658-8501, Japan.; Institute for Integrative Neurobiology, Konan University, Kobe, 658-8501, Japan., Tominaga M; Division of Cell Signaling, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8787, Japan.; Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan., Sokabe T; Division of Cell Signaling, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8787, Japan. sokabe@nips.ac.jp.; Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi, 444-8787, Japan. sokabe@nips.ac.jp., Kuhara A; Graduate School of Natural Science, Konan University, Kobe, 658-8501, Japan. atsushi_kuhara@me.com.; Faculty of Science and Engineering, Konan University, Kobe, 658-8501, Japan. atsushi_kuhara@me.com.; Institute for Integrative Neurobiology, Konan University, Kobe, 658-8501, Japan. atsushi_kuhara@me.com.; AMED-PRIME, Japan Agency for Medical Research and Development, Tokyo, 100-0004, Japan. atsushi_kuhara@me.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Oct 29; Vol. 10 (1), pp. 18566. Date of Electronic Publication: 2020 Oct 29.
DOI: 10.1038/s41598-020-75302-3
Abstrakt: Caenorhabditis elegans (C. elegans) exhibits cold tolerance and temperature acclimatisation regulated by a small number of head sensory neurons, such as the ADL temperature-sensing neurons that express three transient receptor potential vanilloid (TRPV) channel subunits, OSM-9, OCR-2, and OCR-1. Here, we show that an OSM-9/OCR-2 regulates temperature acclimatisation and acts as an accessorial warmth-sensing receptor in ADL neurons. Caenorhabditis elegans TRPV channel mutants showed abnormal temperature acclimatisation. Ectopic expression of OSM-9 and OCR-2 in non-warming-responsive gustatory neurons in C. elegans and Xenopus oocytes revealed that OSM-9 and OCR-2 cooperatively responded to warming; however, neither TRPV subunit alone was responsive to warming. A warming-induced OSM-9/OCR-2-mediated current was detectable in Xenopus oocytes, yet ADL in osm-9 ocr-2 double mutant responds to warming; therefore, an OSM-9/OCR-2 TRPV channel and as yet unidentified temperature receptor might coordinate transmission of temperature signalling in ADL temperature-sensing neurons. This study demonstrates direct sensation of warming by TRPV channels in C. elegans.
Databáze: MEDLINE
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