Emodin inhibited NADPH-quinone reductase competitively and induced cytotoxicity in rat primary hepatocytes.

Autor: Shukla V; Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India., Asthana S; Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, India., Yadav S; Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India., Rajput VS; School of Biotechnology, Jawaharlal Nehru University, Delhi, 110067, India., Tripathi A; Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address: anuragtripathi@iitr.res.in.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2020 Dec; Vol. 188, pp. 117-121. Date of Electronic Publication: 2020 Oct 22.
DOI: 10.1016/j.toxicon.2020.10.018
Abstrakt: Consumption of Cassia occidentalis (CO) seeds, a ubiquitously distributed weed plant, is responsible for a pathological condition known as hepato-myo-encephalopathy (HME). The toxicity of CO seeds is largely attributed to the presence of anthraquinones (AQs). Here, we report that Emodin, a CO anthraquinone, inhibits the enzymatic activity of NADPH-Quinone reductase, which is an intracellular enzyme fundamentally involved in the detoxification of quinone containing compounds. Emodin binds to the active site of the enzyme and acts as a competitive inhibitor with respect to 2, 6-Dichlorophenolindophenol, a known substrate of NADPH-Quinone reductase. Moreover, our in-vitro study further revealed that Emodin was cytotoxic to primary rat hepatocytes.
(Copyright © 2020. Published by Elsevier Ltd.)
Databáze: MEDLINE
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