Comparative mapping and validation of multiple disease resistance QTL for simultaneously controlling common and dwarf bunt in bread wheat.

Autor: Muellner AE; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria.; Saatzucht Donau GesmbH. & CoKG, Saatzuchtstrasse 11, 2301, Probstdorf, Austria., Buerstmayr M; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria. maria.buerstmayr@boku.ac.at., Eshonkulov B; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria.; Samarkand Branch of Tashkent State University of Economics, Professors Street 51, 140147, Samarkand, Usbekistan., Hole D; Utah State University, 2325 Old Main Hill, Logan, UT, 84322, USA., Michel S; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria., Hagenguth JF; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria.; Division of Plant Breeding Methodology, University of Goettingen, Carl-Sprengel-Weg 1, 37075, Göttingen, Germany., Pachler B; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria.; Saatbau Linz eGen, Breeding Station Schoenering, Angerweg 19, 4073, Wilhering, Austria., Pernold R; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria.; Mauerbachstraße 5, 1140, Wien, Austria., Buerstmayr H; Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Konrad Lorenz Straße 20, 3430, Vienna, Tulln, Austria.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2021 Feb; Vol. 134 (2), pp. 489-503. Date of Electronic Publication: 2020 Oct 29.
DOI: 10.1007/s00122-020-03708-8
Abstrakt: Key Message: Resistance QTL on chromosomes 1AL and 7AL are effective against common and dwarf bunt, QTL on 1BS affects common bunt and QTL on 7DS affects dwarf bunt in bread wheat. Common bunt, caused by Tilletia caries and T. laevis, and dwarf bunt, caused by T. controversa, negatively affect grain yield and quality of wheat and are particularly destructive in low-input and organic production systems. Two recombinant inbred line (RIL) populations derived by crossing the highly and durably resistant cultivars 'Blizzard' and 'Bonneville' to the susceptible cultivar 'Rainer' were evaluated for their resistance to common and dwarf bunt in artificially inoculated field and greenhouse trials over two growing seasons and genotyped with a 15 K SNP array. Bunt resistance QTL were mapped to chromosomes 1AL, 1BS, 7AL and 7DS. Common bunt resistance was regulated by the major QTL QBt.ifa-1BS and QBt.ifa-1AL together with the moderate effect QTL QBt.ifa-7AL. Dwarf bunt resistance was on the other hand regulated by the QTL QBt.ifa-1AL, QBt.ifa-7AL and QBt.ifa-7DS. Common bunt resistance QTL exhibited pronounced epistatic effects, while epistatic effects were of smaller magnitude for dwarf bunt QTL. Kompetitive Allele-Specific PCR (KASP) markers were developed from SNPs associated with bunt resistance QTL and successfully used for QTL validation in an independent set of RILs. These KASP markers have the potential to support targeted introgression of QTL into elite wheat germplasm and accelerate breeding for enhanced bunt resistance. Durable protection against both common and dwarf bunt can be achieved by combining multiple resistance genes in the same genetic background.
Databáze: MEDLINE
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