Influence of ultraviolet and gamma ray irradiation on luminescent bacteria and exploring their efficacy as biosensors.

Autor: Wanjari RA; Rajiv Gandhi Biotechnology Center, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India., Shanware AS; Rajiv Gandhi Biotechnology Center, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India., Dhoble SJ; Department of Physics Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2021 Mar; Vol. 36 (2), pp. 525-530. Date of Electronic Publication: 2020 Dec 01.
DOI: 10.1002/bio.3972
Abstrakt: Toxicity monitoring of harmful radiation is an indispensable issue in modern radioecology. As bioluminescent bacteria have the simplest structure to epitomize the biosphere, their bioluminescence can also act as an indicator of the conditions, therefore assay systems based on luminous bacteria can be used to monitor environmental radiotoxicity. The present investigation explored the measurement of bacterial luminescence, which can be easily computed. Bioluminescent bacterial strains were used to evaluate the effect of ultraviolet (UV) and gamma irradiation. A random amplified polymorphic DNA (RAPD) assay was carried out to observe alterations under exposure. Using a phylogenetic tree, a comparative study of the effect of UV and gamma rays was carried out. The isolated strains showed marked sensitivity towards radiation exposure present in the environment and therefore they could be used as potential biosensing elements for developing an on-site pollution monitoring biosensor.
(© 2020 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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