Desert truffle genomes reveal their reproductive modes and new insights into plant-fungal interaction and ectendomycorrhizal lifestyle.

Autor: Marqués-Gálvez JE; Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, 30100, Spain.; INRAE, UMR 1136, Interactions Arbres/Microorganismes (IAM), Centre INRAE GrandEst - Nancy, Université de Lorraine, Champenoux, 54280, France., Miyauchi S; INRAE, UMR 1136, Interactions Arbres/Microorganismes (IAM), Centre INRAE GrandEst - Nancy, Université de Lorraine, Champenoux, 54280, France., Paolocci F; CNR-IBBR, Istituto di Bioscienze e Biorisorse, UOS di Perugia, Perugia, 06128, Italy., Navarro-Ródenas A; Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, 30100, Spain., Arenas F; Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, 30100, Spain., Pérez-Gilabert M; Departamento de Bioquímica y Biología Molecular-A, Universidad de Murcia, Campus de Espinardo, Murcia, 30100, Spain., Morin E; INRAE, UMR 1136, Interactions Arbres/Microorganismes (IAM), Centre INRAE GrandEst - Nancy, Université de Lorraine, Champenoux, 54280, France., Auer L; INRAE, UMR 1136, Interactions Arbres/Microorganismes (IAM), Centre INRAE GrandEst - Nancy, Université de Lorraine, Champenoux, 54280, France., Barry KW; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94598, USA., Kuo A; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94598, USA., Grigoriev IV; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94598, USA.; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, 94598, USA., Martin FM; INRAE, UMR 1136, Interactions Arbres/Microorganismes (IAM), Centre INRAE GrandEst - Nancy, Université de Lorraine, Champenoux, 54280, France., Kohler A; INRAE, UMR 1136, Interactions Arbres/Microorganismes (IAM), Centre INRAE GrandEst - Nancy, Université de Lorraine, Champenoux, 54280, France., Morte A; Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, 30100, Spain.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2021 Mar; Vol. 229 (5), pp. 2917-2932. Date of Electronic Publication: 2020 Dec 10.
DOI: 10.1111/nph.17044
Abstrakt: Desert truffles are edible hypogeous fungi forming ectendomycorrhizal symbiosis with plants of Cistaceae family. Knowledge about the reproductive modes of these fungi and the molecular mechanisms driving the ectendomycorrhizal interaction is lacking. Genomes of the highly appreciated edible desert truffles Terfezia claveryi Chatin and Tirmania nivea Trappe have been sequenced and compared with other Pezizomycetes. Transcriptomes of T. claveryi × Helianthemum almeriense mycorrhiza from well-watered and drought-stressed plants, when intracellular colonizations is promoted, were investigated. We have identified the fungal genes related to sexual reproduction in desert truffles and desert-truffles-specific genomic and secretomic features with respect to other Pezizomycetes, such as the expansion of a large set of gene families with unknown Pfam domains and a number of species or desert-truffle-specific small secreted proteins differentially regulated in symbiosis. A core set of plant genes, including carbohydrate, lipid-metabolism, and defence-related genes, differentially expressed in mycorrhiza under both conditions was found. Our results highlight the singularities of desert truffles with respect to other mycorrhizal fungi while providing a first glimpse on plant and fungal determinants involved in ecto to endo symbiotic switch that occurs in desert truffle under dry conditions.
(©2020 The Authors New Phytologist ©2020 New Phytologist Foundation.)
Databáze: MEDLINE
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