Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model.

Autor: Boteanu RM; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania., Suica VI; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania., Ivan L; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania., Safciuc F; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania., Uyy E; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania., Dragan E; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania., Croitoru SM; Faculty of Engineering and Management of Technological Systems, Politehnica University of Bucharest, Bucharest, Romania., Grumezescu V; National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele, P.O. Box MG-54, 77125, Bucharest, Romania., Chiritoiu M; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania., Sima LE; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania., Vlagioiu C; Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania., Socol G; National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, Magurele, P.O. Box MG-54, 77125, Bucharest, Romania. gabriel.socol@inflpr.ro., Antohe F; Institute of Cellular Biology and Pathology 'N. Simionescu' of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania. felicia.antohe@icbp.ro.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Oct 28; Vol. 10 (1), pp. 18493. Date of Electronic Publication: 2020 Oct 28.
DOI: 10.1038/s41598-020-75527-2
Abstrakt: Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and in a rat tibial defect model (100% viability). Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. The proposed functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.
Databáze: MEDLINE
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