Human and mouse bones physiologically integrate in a humanized mouse model while maintaining species-specific ultrastructure.

Autor:	Moreno-Jiménez I; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany.; Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, Australia., Cipitria A; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany., Sánchez-Herrero A; Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, Australia., van Tol AF; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany., Roschger A; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany., Lahr CA; Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, Australia., McGovern JA; Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, Australia., Hutmacher DW; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany. dietmar.hutmacher@qut.edu.au peter.fratzl@mpikg.mpg.de.; Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, Australia., Fratzl P; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany. dietmar.hutmacher@qut.edu.au peter.fratzl@mpikg.mpg.de.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2020 Oct 28; Vol. 6 (44). Date of Electronic Publication: 2020 Oct 28 (Print Publication: 2020).
DOI:	10.1126/sciadv.abb9265
Abstrakt:	Humanized mouse models are increasingly studied to recapitulate human-like bone physiology. While human and mouse bone architectures differ in multiple scales, the extent to which chimeric human-mouse bone physiologically interacts and structurally integrates remains unknown. Here, we identify that humanized bone is formed by a mosaic of human and mouse collagen, structurally integrated within the same bone organ, as shown by immunohistochemistry. Combining this with materials science techniques, we investigate the extracellular matrix of specific human and mouse collagen regions. We show that human-like osteocyte lacunar-canalicular network is retained within human collagen regions and is distinct to that of mouse tissue. This multiscale analysis shows that human and mouse tissues physiologically integrate into a single, functional bone tissue while maintaining their species-specific ultrastructural differences. These results offer an original method to validate and advance tissue-engineered human-like bone in chimeric animal models, which grow to be eloquent tools in biomedical research. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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