| Autor: |
Li Y; Department of Haematology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China., Qiu Y; Department of Spine Surgery, The Second Affiliated Hospital of Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong, China., Ye C; Department of Dermato-Venereology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510640, China., Chen L; School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou, 510640, China., Liang Y; Guangdong Zhongqing Font Biochemical Science and Technology Co. Ltd., Maoming, 525427, Guangdong, China., Huang TY; Department of Laboratory Medicine, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China., Zhang L; Department of Haematology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China., Liu J; School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou, 510640, China. yaner0722@hotmail.com. |
| Abstrakt: |
Staphylococcus aureus (S. aureus) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by S. aureus contaminated food has become a global health problem. S. aureus can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in S. aureus is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in S. aureus, including sea, seb, sec, see, pvl and tsst, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in S. aureus. |
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