Processing Human Thymic Tissue for Single Cell RNA-Seq.
| Autor: | Le J; Cancer and Blood Disease Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA., Ha VL; Cancer and Blood Disease Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.; Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA., Luong A; Cancer and Blood Disease Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA., Parekh C; Cancer and Blood Disease Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2020 Aug 24; Vol. 1 (2), pp. 100090. Date of Electronic Publication: 2020 Aug 24 (Print Publication: 2020). |
| DOI: | 10.1016/j.xpro.2020.100090 |
| Abstrakt: | Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34 + progenitor and more differentiated CD34 - fractions from post-natal thymic tissue to study thymopoiesis. CD34 + cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34 + cells. For complete details on the use and execution of this protocol, please refer to Le et al. (2020). Competing Interests: The authors declare no competing interests. (© 2020 The Authors.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|