Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model.

Autor:	Gedar Totuk ÖM; Department of Ophthalmology, Bahçeşehir University Faculty of Medicine, İstanbul-Turkey., Güzel ŞE; Department of Orthopedics and Traumatology, T.C. Ministry of Health Adıyaman Kahta State Hospital, Adıyaman-Turkey., Ekici H; Department of Pharmacology and Toxicology, Kırıkkale University Faculty of Veterinary Medicine, Kırıkkale-Turkey., Kumandaş A; Department of Surgery, Kırıkkale University Faculty of Veterinary Medicine, Kırıkkale-Turkey., Emre Aydıngöz S; Department of Pharmacology, Başkent University Faculty of Medicine, Ankara-Turkey., Yılmaz EÇ; Bahçeşehir University Faculty of Medicine, 4rd Grade Student, İstanbul-Turkey., Kırdan T; Bahçeşehir University Faculty of Medicine, 5th Grade Student, İstanbul-Turkey., Midi A; Department of Pathology, Bahçeşehir University Faculty of Medicine, İstanbul-Turkey.
Jazyk:	angličtina
Zdroj:	Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES [Ulus Travma Acil Cerrahi Derg] 2020 Nov; Vol. 26 (6), pp. 853-858.
DOI:	10.14744/tjtes.2020.50384
Abstrakt:	Background: Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model. Methods: Forty-eight Sprague Dawley rats (5-7 weeks old, 180-210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped. Results: Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0±1.2 seconds and 8.0±1.3 seconds in heparinized and non-heparinized groups, respectively. Conclusion: AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.
Databáze:	MEDLINE
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