| Autor: |
Molitor DCA; Institute of Pathology, University Hospital Bonn, Bonn, Germany., Boor P; Institute of Pathology, University Hospital Aachen, RWTH Aachen, Bonn, Germany., Buness A; Institute for Medical Biometry, Informatics and Epidemiology, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.; Institute for Genomic Statistics and Bioinformatics, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany., Schneider RK; Department of Hematology, Erasmus MC Cancer Center, Rotterdam, Netherlands.; Institute for Biomedical Engineering Department of Cell Biology , RWTH , Aachen, Germany., Teichmann LL; Department of Hematology and Oncology, University Hospital Bonn, Bonn, Germany., Körber RM; Department of Hematology and Oncology, University Hospital Bonn, Bonn, Germany., Horvath GL; Medical Faculty, Microscopy Core Facility, University of Bonn, Bonn, Germany., Koschmieder S; Department of Hematology, Oncology, Hemostaseology, and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen, Aachen, Germany., Gütgemann I; Institute of Pathology, University Hospital Bonn, Bonn, Germany. Ines.Guetgemann@ukbonn.de. |
| Abstrakt: |
Bone marrow (BM) fibrosis in myeloproliferative neoplasms (MPNs) is associated with a poor prognosis. The development of myelofibrosis and differentiation of mesenchymal stromal cells to profibrotic myofibroblasts depends on macrophages. Here, we compared macrophage frequencies in BM biopsies of MPN patients and controls (patients with non-neoplastic processes), including primary myelofibrosis (PMF, n = 18), essential thrombocythemia (ET, n = 14), polycythemia vera (PV, n = 12), and Philadelphia chromosome-positive chronic myeloid leukemia (CML, n = 9). In PMF, CD68-positive macrophages were greatly increased compared to CML (p = 0.017) and control BM (p < 0.001). Similar findings were observed by CD163 staining (PMF vs. CML: p = 0.017; PMF vs. control: p < 0.001). Moreover, CD68-positive macrophages were increased in PV compared with ET (p = 0.009) and reactive cases (p < 0.001). PMF had higher frequencies of macrophages than PV (CD68: p < 0.001; CD163: p < 0.001) and ET (CD68: p < 0.001; CD163: p < 0.001). CD163 and CD68 were often co-expressed in macrophages with stellate morphology in Philadelphia chromosome-negative MPN, resulting in a sponge-like reticular network that may be a key regulator of unbalanced hematopoiesis in the BM space and may explain differences in cellularity and clinical course. |
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