Pathogenicity of severe fever with thrombocytopenia syndrome virus in mice regulated in type I interferon signaling: Severe fever with thrombocytopenia and type I interferon.

Autor: Park SC; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea., Park JY; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea.; Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54531 Republic of Korea., Choi JY; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea., Lee SG; Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54531 Republic of Korea., Eo SK; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea.; Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54531 Republic of Korea., Oem JK; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea., Tark DS; Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54531 Republic of Korea., You M; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea., Yu DH; College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828 Republic of Korea., Chae JS; Laboratory of Veterinary Internal Medicine, BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826 Republic of Korea., Kim B; College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596 Republic of Korea.; Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54531 Republic of Korea.
Jazyk: angličtina
Zdroj: Laboratory animal research [Lab Anim Res] 2020 Oct 21; Vol. 36, pp. 38. Date of Electronic Publication: 2020 Oct 21 (Print Publication: 2020).
DOI: 10.1186/s42826-020-00070-0
Abstrakt: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease, which causes high fever, thrombocytopenia, and death in humans and animals in East Asian countries. The pathogenicity of SFTS virus (SFTSV) remains unclear. We intraperitoneally infected three groups of mice: wild-type (WT), mice treated with blocking anti-type I interferon (IFN)-α receptor antibody (IFNAR Ab), and IFNAR knockout (IFNAR -/- ) mice, with four doses of SFTSV (KH1, 5 × 10 5 to 5 × 10 2 FAID 50 ). The WT mice survived all SFTSV infective doses. The IFNAR Ab mice died within 7 days post-infection (dpi) with all doses of SFTSV except that the mice were infected with 5 × 10 2 FAID 50 SFTSV. The IFNAR -/- mice died after infection with all doses of SFTSV within four dpi. No SFTSV infection caused hyperthermia in any mice, whereas all the dead mice showed hypothermia and weight loss. In the WT mice, SFTSV RNA was detected in the eyes, oral swabs, urine, and feces at 5 dpi. Similar patterns were observed in the IFNAR Ab and IFNAR -/- mice after 3 dpi, but not in feces. The IFNAR Ab mice showed viral shedding until 7 dpi. The SFTSV RNA loads were higher in organs of the IFNAR -/- mice compared to the other groups. Histopathologically, coagulation necrosis and mononuclear inflammatory cell infiltration in the liver and white pulp atrophy in the spleen were seen as the main lesions in the IFN signaling lacking mice. Immunohistochemically, SFTSV antigens were mainly detected in the marginal zone of the white pulp of the spleen in all groups of mice, but more viral antigens were observed in the spleen of the IFNAR -/- mice. Collectively, the IFN signaling-deficient mice were highly susceptible to SFTSV and more viral burden could be demonstrated in various excreta and organs of the mice when IFN signaling was inhibited.
Competing Interests: Competing interestsThe authors have no conflict of interest to report.
(© The Author(s) 2020.)
Databáze: MEDLINE
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