| Autor: |
Fernández-Juárez V; Marine Ecology and Systematics (MarES), Department of Biology, University of the Balearic Islands, Palma, Spain., Bennasar-Figueras A; Grup de Microbiologia, Department of Biology, University of the Balearic Islands, Palma, Spain., Sureda-Gomila A; Research Group on Community Nutrition and Oxidative Stress, University of the Balearic Islands and CIBEROBN (Fisiopatología de la Obesidad y la Nutrición), Palma, Spain., Ramis-Munar G; Cellomic Unit of University Institute of Research in Health Sciences of the Balearic Islands, Palma, Spain., Agawin NSR; Marine Ecology and Systematics (MarES), Department of Biology, University of the Balearic Islands, Palma, Spain. |
| Jazyk: |
angličtina |
| Zdroj: |
Frontiers in microbiology [Front Microbiol] 2020 Sep 25; Vol. 11, pp. 541558. Date of Electronic Publication: 2020 Sep 25 (Print Publication: 2020). |
| DOI: |
10.3389/fmicb.2020.541558 |
| Abstrakt: |
Diazotrophs or N 2 -fixers are one of the most ecologically significant groups in marine ecosystems (pelagic and benthic). Inorganic phosphorus (PO 4 3- ) and iron (Fe) can limit the growth and N 2 -fixing capacities of cyanobacteria. However, studies investigating co-limitation of these factors are lacking. Here, we added different concentrations of PO 4 3- and Fe in two cyanobacterial species whose relatives can be found in seagrass habitats: the unicellular Halothece sp. (PCC 7418) and the filamentous Fischerella muscicola (PCC 73103), grown under different nitrate (NO 3 - ) concentrations and under N 2 as sole N source, respectively. Their growth, pigment content, N 2 -fixation rates, oxidative stress responses, and morphological and cellular changes were investigated. Our results show a serial limitation of NO 3 - and PO 4 3- (with NO 3 - as the primary limiting nutrient) for Halothece sp. Simultaneous co-limitation of PO 4 3- and Fe was found for both species tested, and high levels of Fe (especially when added with high PO 4 3- levels) inhibited the growth of Halothece sp. Nutrient limitation (PO 4 3- , Fe, and/or NO 3 - ) enhanced oxidative stress responses, morphological changes, and apoptosis. Furthermore, an extensive bio-informatic analysis describing the predicted Pho, Fur, and NtcA regulons (involved in the survival of cells to P, Fe, and N limitation) was made using the complete genome of Halothece sp. as a model, showing the potential of this strain to adapt to different nutrient regimes (P, Fe, or N).
(Copyright © 2020 Fernández-Juárez, Bennasar-Figueras, Sureda-Gomila, Ramis-Munar and Agawin.) |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
