Isolation and characterization of microsatellite loci in merlins (Falco columbarius) and cross-species amplification in gyrfalcons (F. rusticolus) and peregrine falcons (F. peregrinus).

Autor: Hull JM; Department of Animal Science, University of California, Davis, 1 Shields Avenue, Davis, CA, 95616, USA.; Golden Gate Raptor Observatory, Building 1064 Fort Cronkhite, Sausalito, CA, 94965, USA., Sage GK; U.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA., Sonsthagen SA; U.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA., Gravley MC; U.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA. mgravley@usgs.gov., Martinico BL; Department of Animal Science, University of California, Davis, 1 Shields Avenue, Davis, CA, 95616, USA., Booms TL; Alaska Department of Fish and Game, Threatened, Endangered and Diversity Program, 1300 College Road, Fairbanks, AK, 99701, USA., Swem T; U.S. Fish and Wildlife Service, Fairbanks Fish & Wildlife Field Office, 101 12th Avenue, Fairbanks, AK, 99701, USA., Talbot SL; U.S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2020 Nov; Vol. 47 (11), pp. 8377-8383. Date of Electronic Publication: 2020 Oct 24.
DOI: 10.1007/s11033-020-05842-4
Abstrakt: Merlins, Falco columbarius, breed throughout temperate and high latitude habitats in Asia, Europe, and North America. Like peregrine falcons, F. peregrinus, merlins underwent population declines during the mid-to-late twentieth century, due to organochlorine-based contamination, and have subsequently recovered, at least in North American populations. To better understand levels of genetic diversity and population structuring in contemporary populations and to assess the impact of the twentieth century decline, we used genomic data archived in public databases and constructed genomic libraries to isolate and characterize a suite of 17 microsatellite markers for use in merlins. We also conducted cross-amplification experiments to determine the markers' utility in peregrine falcons and gyrfalcons, F. rusticolus. These markers provide a valuable addition to marker suites that can be used to determine individual identity and conduct genetic analyses on merlins and congeners.
Databáze: MEDLINE