| Autor: |
Klobučar M; University of Rijeka, Department of Biotechnology, Centre for high-throughput technologies, Radmile Matejčić 2, 51000 Rijeka, Croatia., Pavlić SD; University of Rijeka, Department of Medical Biology and Genetics, Faculty of Medicine, B. Branchetta 20, 51000 Rijeka, Croatia., Car I; University of Rijeka, Department of Biotechnology, Centre for high-throughput technologies, Radmile Matejčić 2, 51000 Rijeka, Croatia., Severinski NS; Department of Obstetrics and Gynaecology, Clinical Hospital Centre Rijeka, Cambierieva 17/5, 51000 Rijeka, Croatia., Milaković TT; Department of Obstetrics and Gynaecology, Clinical Hospital Centre Rijeka, Cambierieva 17/5, 51000 Rijeka, Croatia., Badovinac AR; University of Rijeka, Department of Biotechnology, Centre for high-throughput technologies, Radmile Matejčić 2, 51000 Rijeka, Croatia.; University of Rijeka, Department of Medical Biology and Genetics, Faculty of Medicine, B. Branchetta 20, 51000 Rijeka, Croatia., Pavelić SK; University of Rijeka Faculty of Health Studies, Viktora Cara Emina 5, 51000 Rijeka, Croatia. |
| Abstrakt: |
Couples with infertility issues have been assisted by in vitro fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of in vitro fertilization procedures, but other processes are also critical for fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing fertilization through either (1) assisted reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients. |
