Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor.

Autor:	Oseev A; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France., Mukhin N; Institute for Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, 39106 Magdeburg, Germany., Elie-Caille C; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France., Boireau W; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France., Lucklum R; Institute for Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, 39106 Magdeburg, Germany., Lecompte T; Geneva Platelet Group, Faculty of Medicine, Geneva University, 1205 Geneva, Switzerland.; Haemostasis Unit, Department of Medical Specialities, University Hospital Geneva (HUG), 1205 Geneva, Switzerland., Remy-Martin F; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France., Manceau JF; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France., Chollet F; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France., Leblois T; FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France.
Jazyk:	angličtina
Zdroj:	Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2020 Oct 21; Vol. 10 (10). Date of Electronic Publication: 2020 Oct 21.
DOI:	10.3390/nano10102079
Abstrakt:	Shear bulk acoustic type of resonant biosensors, such as the quartz crystal microbalance (QCM), give access to label-free in-liquid analysis of surface interactions. The general understanding of the sensing principles was inherited from past developments in biofilms measurements and applied to cells while keeping the same basic assumptions. Thus, the biosensor readouts are still quite often described using 'mass' related terminology. This contribution aims to show that assessment of cell deposits with acoustic biosensors requires a deep understanding of the sensor transduction mechanism. More specifically, the cell deposits should be considered as a structured viscoelastic load and the sensor response depends on both material and topological parameters of the deposits. This shifts the paradigm of acoustic biosensor away from the classical mass loading perspective. As a proof of the concept, we recorded QCM frequency shifts caused by blood platelet deposits on a collagen surface under different rheological conditions and observed the final deposit shape with atomic force microscopy (AFM). The results vividly demonstrate that the frequency shift is highly impacted by the platelet topology on the bio-interface. We support our findings with numerical simulations of viscoelastic unstructured and structured loads in liquid. Both experimental and theoretical studies underline the complexity behind the frequency shift interpretation when acoustic biosensing is used with cell deposits.
Databáze:	MEDLINE
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