Serovar-dependent differences in Hfq-regulated phenotypes in Actinobacillus pleuropneumoniae.

Autor:	Crispim JS; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil., da Silva TF; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil., Sanches NM; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil., da Silva GC; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil., Pereira MF; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil., Rossi CC; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil., Li Y; Section of Paediatric Infectious Disease, Imperial College London, St Mary's Campus, London W2 1PG, UK., Terra VS; Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK., Vohra P; Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK., Wren BW; Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK., Langford PR; Section of Paediatric Infectious Disease, Imperial College London, St Mary's Campus, London W2 1PG, UK., Bossé JT; Section of Paediatric Infectious Disease, Imperial College London, St Mary's Campus, London W2 1PG, UK., Bazzolli DMS; Laboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa, Viçosa, 36570-900, Brazil.
Jazyk:	angličtina
Zdroj:	Pathogens and disease [Pathog Dis] 2020 Nov 23; Vol. 78 (9).
DOI:	10.1093/femspd/ftaa066
Abstrakt:	The RNA chaperone Hfq regulates diverse processes in numerous bacteria. In this study, we compared phenotypes (growth rate, adherence, response to different stress conditions and virulence in Galleria mellonella) of wild-type (WT) and isogenic hfq mutants of three serovars (1, 8 and 15) of the porcine pathogen Actinobacillus pleuropneumoniae. Similar growth in rich broth was seen for all strains except Ap1∆hfq, which showed slightly reduced growth throughout the 24 h time course, and the complemented Ap8∆hfqC mutant had a prolonged lag phase. Differences were seen between the three serovar WT strains regarding adherence, stress response and virulence in G. mellonella, and deletion of hfq affected some, but not all of these phenotypes, depending on serovar. Complementation by expression of cloned hfq from an endogenous promoter only restored some WT phenotypes, indicating that complex regulatory networks may be involved, and that levels of Hfq may be as important as presence/absence of the protein regarding its contribution to gene regulation. Our results support that Hfq is a pleiotropic global regulator in A. pleuropneumoniae, but serovar-related differences exist. These results highlight the importance of testing multiple strains/serovars within a given species when determining contributions of global regulators, such as Hfq, to expression of complex phenotypes. (© The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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