The association between cagL and cagA, vacAs-m, babA genes in patients with gastric cancer, duodenal ulcer, and non-ulcer dyspepsia related to Helicobacter pylori.

Autor:	Demiryas S; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of General Surgery, Istanbul, Turkey., Caliskan R; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Saribas S; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Akkus S; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Gareayaghi N; Istanbul Sisli Hamidiye Etfal Training and Research Hospital, Blood Center, University of Health Sciences, Istanbul, Turkey., Kirmusaoglu S; T.C. Haliç University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey., Kepil N; Istanbul University- Cerrahpasa, Cerrahpasa Medical Faculty, Department of Pathology, Istanbul, Turkey., Dinc H; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Dag H; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey., Dagdeviren E; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey., Tokman HB; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Kalayci F; Istanbul Yeni Yuzyil University, Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Demirci M; Beykent University Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey., Tasci I; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of General Surgery, Istanbul, Turkey., Erzin Y; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Gastroenterology, Istanbul, Turkey., Bal K; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Gastroenterology, Istanbul, Turkey., Kocazeybek B; Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey.
Jazyk:	angličtina
Zdroj:	Acta gastro-enterologica Belgica [Acta Gastroenterol Belg] 2020 Jul-Sep; Vol. 83 (3), pp. 385-392.
Abstrakt:	Introduction: As a component of the cag T4SS, the cagL gene is involved in the translocation of CagA into host cells and is essential for the formation of cag PAI-associated pili between H. pylori and gastric epithelial cells. Aim: We aimed to investigate the clinical association of the cagL gene with other virulence factors (VacA, CagA, EPIYA-C, and BabA protein) of H. pylori strains isolated from GC, duodenal ulcer (DU), and non-ulcer dyspepsia (NUD) cases. Methods: The patient group (PG), including 47 patients (22 GC and 25 DU) and a 25 control group (CG= NUD) were included. Amplification of the H. pylori cagL, cagA, vacA, and babA2 genes and typing of EPIYA motifs were performed by PCR methods. Results: Sixty-one (84.7%) H. pylori strains were detected with cagL (93.6% in SG, 68% in CG). We detected a significant difference between SG and CG for the presence of cagL (p=0.012) but no statistical comparison was done for (≥2) EPIYA-C repeats In the comparison of H. pylori strains with cagA/vacAs1m1 and cagA/ vacAs1m2 and babA2 for the presence of cagL, we could not detect a significant difference (p=1). Conclusion: We detected a significant difference between groups for the presence of cagL genotype (p=0.012). The vacAs1m1 (OR: 2.829), genotypes increased the GC and DU risk by 2.8 times, while multiple (≥2) EPIYA-C repeats incresed the GC and DU risk by 3.524 times. Gender (to be female) (OR: 0.454) decreased the GC and DU risk by inversly decreased in the multivariate analysis. Competing Interests: The authors declare that they have no conflict of interest (© Acta Gastro-Enterologica Belgica.)
Databáze:	MEDLINE
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