Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew ( Taxus wallichiana ) in Nepal.
| Autor: | Gauchan DP; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., Kandel P; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., Tuladhar A; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., Acharya A; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., Kadel U; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., Baral A; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., Shahi AB; Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Kavrepalanchok District, Bagmati, 45200, Nepal., García-Gil MR; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, SE-901 83, Sweden. |
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| Jazyk: | angličtina |
| Zdroj: | F1000Research [F1000Res] 2020 May 19; Vol. 9, pp. 379. Date of Electronic Publication: 2020 May 19 (Print Publication: 2020). |
| DOI: | 10.12688/f1000research.23250.1 |
| Abstrakt: | Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties. Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts. Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia . The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternata , Cladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC Competing Interests: No competing interests were disclosed. (Copyright: © 2020 Gauchan DP et al.) |
| Databáze: | MEDLINE |
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