Cytotoxic, Antitumor and Toxicological Profile of Passiflora alata Leaf Extract.

Autor: Amaral RG; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe 49100-000, Brazil., Gomes SVF; Institute of Technology and Research, University of Tiradentes, Aracaju, Sergipe 49032-490, Brazil., Andrade LN; Department of Medicine, Federal University of Sergipe (UFS), Avenida Governador Marcelo Déda, Lagarto-SE 49400-000, Brazil., Dos Santos SA; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe 49100-000, Brazil., Severino P; Institute of Technology and Research, University of Tiradentes, Aracaju, Sergipe 49032-490, Brazil.; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.; Tiradentes Institute, 150 Mt Vernon St, Dorchester, MA 02125, USA., de Albuquerque Júnior RLC; Institute of Technology and Research, University of Tiradentes, Aracaju, Sergipe 49032-490, Brazil., Souto EB; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.; CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal., Brandão GC; Department of Pharmacy, Federal University of Ouro Preto, Ouro Preto 78950-000, Brazil., Santos SL; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe 49100-000, Brazil., David JM; Institute of Chemistry, Federal University of Bahia, Salvador 40000-000, Brazil., Carvalho AA; Department of Medicine, Federal University of Sergipe (UFS), Avenida Governador Marcelo Déda, Lagarto-SE 49400-000, Brazil.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2020 Oct 20; Vol. 25 (20). Date of Electronic Publication: 2020 Oct 20.
DOI: 10.3390/molecules25204814
Abstrakt: Passiflora alata or passion fruit is a native flowering plant from Amazon, geographically spread from Peru to Brazil. The plant has long been used in folks medicine for its pharmacological properties and is included in the Brazilian Pharmacopoeia since 1929. The aim of this study was to evaluate the potential cytotoxic and antitumor activities of Passiflora alata leaf extract (P a LE) in S180-tumor bearing mice. The percentage of cell proliferation inhibition (% CPI) and IC 50 in relation to 4 tumor cell lines were determined in PC3, K-562, HepG2 and S180 cell lines using the MTT assay. P a LE showed a CPI > 75% and greater potency (IC 50 < 30 µg/mL) against PC3 and S180 cell lines. Pa LE showed antitumor activity in treatments intraperitoneally (36.75% and 44.99% at doses of 100 and 150 mg/kg/day, respectively). Toxicological changes were shown in the reduced body mass associated with reduced food consumption, increased spleen mass associated with histopathological increase in the white pulp of the spleen and increased number of total leukocytes with changes in the percentage relationship between lymphocytes and neutrophils. Our outcomes corroborate the conclusion that Pa LE has antitumor activity in vitro and in vivo with low toxicity.
Databáze: MEDLINE
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