Antigen-specific memory and naïve CD4+ T cells following secondary Chlamydia trachomatis infection.

Autor: Helble JD; Department of Microbiology, Harvard Medical School, Boston, MA, United States of America., Mann AO; Department of Immunology, Harvard Medical School, Boston, MA, United States of America., Starnbach MN; Department of Microbiology, Harvard Medical School, Boston, MA, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Oct 22; Vol. 15 (10), pp. e0240670. Date of Electronic Publication: 2020 Oct 22 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0240670
Abstrakt: Memory antigen-specific CD4+ T cells against Chlamydia trachomatis are necessary for protection against secondary genital tract infection. While it is known that naïve antigen-specific CD4+ T cells can traffic to the genital tract in an antigen-specific manner, these T cells are not protective during primary infection. Here, we sought to compare the differences between memory and naïve antigen-specific CD4+ T cells in the same mouse following secondary infection using transgenic CD4+ T cells (NR1 T cells). Using RNA sequencing, we found that there were subtle but distinct differences between these two T cell populations. Naïve NR1 T cells significantly upregulated cell cycle genes and were more proliferative than memory NR1 T cells in the draining lymph node. In contrast, memory NR1 T cells were more activated than naïve NR1 T cells and were enriched in the genital tract. Together, our data provide insight into the differences between memory and naïve antigen-specific CD4+ T cells during C. trachomatis infection.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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