Effect of pH on the activity of ice-binding protein from Marinomonas primoryensis.

Autor: Delesky EA; Materials Science and Engineering Program, University of Colorado Boulder, Boulder, CO, 80309, USA., Thomas PE; Department of Biochemistry, University of Colorado Boulder, Boulder, CO, 80303, USA.; Renewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80309, USA., Charrier M; Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO, 80309, USA., Cameron JC; Department of Biochemistry, University of Colorado Boulder, Boulder, CO, 80303, USA.; Renewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80309, USA.; National Renewable Energy Laboratory, Golden, CO, 80401, USA., Srubar WV 3rd; Materials Science and Engineering Program, University of Colorado Boulder, Boulder, CO, 80309, USA. wsrubar@colorado.edu.; Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO, 80309, USA. wsrubar@colorado.edu.
Jazyk: angličtina
Zdroj: Extremophiles : life under extreme conditions [Extremophiles] 2021 Jan; Vol. 25 (1), pp. 1-13. Date of Electronic Publication: 2020 Oct 22.
DOI: 10.1007/s00792-020-01206-9
Abstrakt: The ability of an ice-binding protein (IBP) from Marinomonas primoryensis (MpIBP) to influence ice crystal growth and structure in nonphysiological pH environments was investigated in this work. The ability for MpIBP to retain ice interactivity under stressed environmental conditions was determined via (1) a modified splat assay to determine ice recrystallization inhibition (IRI) of polycrystalline ice and (2) nanoliter osmometry to evaluate the ability of MpIBP to dynamically shape the morphology of a single ice crystal. Circular dichroism (CD) was used to relate the IRI and DIS activity of MpIBP to secondary structure. The results illustrate that MpIBP secondary structure was stable between pH 6 and pH 10. It was found that MpIBP did not interact with ice at pH ≤ 4 or pH ≥ 13. At 6 ≤ pH ≥ 12 MpIBP exhibited a reduction in grain size of ice crystals as compared to control solutions and demonstrated dynamic ice shaping at 6 ≤ pH ≥ 10. The results substantiate that MpIBP retains some secondary structure and function in non-neutral pH environments; thereby, enabling its potential utility in nonphysiological materials science and engineering applications.
Databáze: MEDLINE
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