Proteomics analysis identified TPI1 as a novel biomarker for predicting recurrence of intrahepatic cholangiocarcinoma.

Autor: Yu WL; Department II of Biliary Tract Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China., Yu G; Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, NO.725, South Wanping RoadShanghai, Shanghai, 200032, China., Dong H; Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China., Chen K; Department of Pathology, Zhong Shan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China., Xie J; The School of Life Science, Fudan University, 2005 Songhu Road, 200438, Shanghai, China., Yu H; Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China., Ji Y; Department of Pathology, Zhong Shan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China., Yang GS; Department of Hepatic Surgery, Shanghai Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China., Li AJ; Department of Special Treatment II, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China. ajLihappy@163.com., Cong WM; Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China. wmcong@smmu.edu.cn., Jin GZ; Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111, Xianxia Road, Shanghai, 200336, China. jgzhi@hotmail.com.
Jazyk: angličtina
Zdroj: Journal of gastroenterology [J Gastroenterol] 2020 Dec; Vol. 55 (12), pp. 1171-1182. Date of Electronic Publication: 2020 Oct 21.
DOI: 10.1007/s00535-020-01729-0
Abstrakt: Background: Intrahepatic cholangiocarcinoma (ICC) is the second most common tumor in primary liver cancer, but the prognostic factors associated with long-term outcomes after surgical resection remain poorly defined. This study aimed to develop a novel prognostic classifier for patients with ICC after surgery.
Methods: Using a proteomics approach, we screened tumor markers that up-regulated in ICC tissues, and narrowed down by bioinformatics analysis, western blot and immunohistochemistry. Prognostic markers were identified using Cox regression analyses in primary training cohort and the predictive models for time to recurrence (TTR) were established. The predictive accuracy of predictive model was validated in external validation cohort and prospective validation cohort. MTT assay, clonal formation assay and trans-well assays were used to verify the effect on the proliferation and migration in ICC cell line.
Results: Triosephosphate isomerise (TPI1) was significantly up-regulated in ICC tissues and Kaplan-Meier analysis reveals that higher TPI1 expression was strongly correlated with higher recurrence rate of ICC patients. In the primary training cohort, mean TTR was significantly longer (p < 0.0001) than in the low-risk group (26.9 months for TTR, 95% CI 22.4-31.5) than in the high-risk group (14.5 months for TTR, 95% CI 10.6-18.4). Similar results were observed in two validation cohorts. In addition, a nomogram to predict recurrence was developed. Moreover, Knockdown of TPI1 by shRNA inhibited ICC cell growth, colony information, migration, invasion in vitro.
Conclusions: Current prognostic models were accurate in predicting recurrence for ICC patients after surgical resection.
Databáze: MEDLINE
Externí odkaz: