Oral shedding of CMV and HSV-1 in hematopoietic stem cell transplantation patients.

Autor: Costa ALF; Postgraduate Program in Dentistry, Cruzeiro Do Sul University (UNICSUL), São Paulo, Brazil., Santos BA; Hematology and Hemotherapy Center, University of Campinas (UNICAMP), Campinas, Brazil., Torregrossa VR; Hematology and Hemotherapy Center, University of Campinas (UNICAMP), Campinas, Brazil., Miranda ECM; Hematology and Hemotherapy Center, University of Campinas (UNICAMP), Campinas, Brazil., Vigorito AC; Hematology and Hemotherapy Center, University of Campinas (UNICAMP), Campinas, Brazil., Palmieri M; Department of Stomatology, School of Dentistry, University of São Paulo (USP), São Paulo, Brazil., Ricardo ALF; Postgraduate Program in Dentistry, Cruzeiro Do Sul University (UNICSUL), São Paulo, Brazil., Sarmento DJS; Department of Stomatology, School of Dentistry, University of São Paulo (USP), São Paulo, Brazil.; Department of Stomatology, School of Dentistry, State University of Paraíba, Araruna, Brazil., Mamana AC; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo (USP), São Paulo, Brazil., Tozetto-Mendoza TR; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo (USP), São Paulo, Brazil., Correa MEP; Hematology and Hemotherapy Center, University of Campinas (UNICAMP), Campinas, Brazil., Braz-Silva PH; Department of Stomatology, School of Dentistry, University of São Paulo (USP), São Paulo, Brazil.; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo (USP), São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2021 Sep; Vol. 27 (6), pp. 1572-1579. Date of Electronic Publication: 2020 Nov 08.
DOI: 10.1111/odi.13690
Abstrakt: Objectives: To evaluate the oral shedding of herpesviruses in patients undergoing hematopoietic stem cell transplantation (HSCT) and correlate it with oral mucositis (OM).
Methods: Saliva samples were collected before the HSCT and on day D + 8. Multiplex Polymerse Chain Reaction (PCR) was performed to detect herpes simplex virus (HSV)-1 and HSV-2, Epstein-Barr virus (EBV), Cytomegalovirus (CMV), Variella-zoster virus (VZV), and human herpesvirus (HHV)-6, HHV-7, and HHV-8. OM was assessed according to WHO criteria.
Results: Thirty one patients were enrolled, in which 20 of 31 (64.5%) were males; median age was 50 (21-70) years; 16 of 31 (51.6%) underwent allo-HSCT; and 15 of 31 (48.4%) underwent auto-HSCT. On D + 8, OM grades III and IV were observed in 8 of 31 (25.8%) patients. In the first salivary collection, EBV was found in 24 of 31 (77.4%), followed by HHV-6 (7/31, 22.6%) and HHV-7 (8/31 25.8%). In the second collection, EBV was found in 24 of 27(89%), followed by HSV-1 (8/27, 30%) and CMV, HHV-6, and HHV-7 (5/27, 18.5%, each one). On D + 8, OM grades II and IV were associated with the presence of HSV-1. HSV-1 was also associated with worsening degrees of OM on D + 15.
Conclusion: The presence of HSV-1 and CMV in oral samples was more frequent on day D + 8 after HSCT. HSV-1 detection was associated with severity and worsening of OM. HSV-1 and CMV seem to be associated with oral dysbiosis due to HSCT.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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