Molecular characteristics of rotavirus genotypes circulating in the south of Benin, 2016-2018.

Autor: Agbla JM; Ministry of Public Health, National Health Laboratory, 01 P.O. Box 418, Cotonou, Benin.; Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin., Esona MD; Viral Gastroenteritis Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control CDC, 1600 Clifton Road, NE, Atlanta, GA, 30329, USA. mdi4@cdc.gov., Agbankpe AJ; Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin., Capo-Chichi A; Epidemiological Surveillance Service, Ministry of Public Health, 01 P.O. Box 418, Cotonou, Benin., Gautam R; Viral Gastroenteritis Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control CDC, 1600 Clifton Road, NE, Atlanta, GA, 30329, USA., Dougnon TV; Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin., Razack O; Central Clinic of Abomey Calavi, 01 P.O. Box 418, Cotonou, Benin., Bowen MD; Viral Gastroenteritis Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control CDC, 1600 Clifton Road, NE, Atlanta, GA, 30329, USA., Bankole HS; Ministry of Public Health, National Health Laboratory, 01 P.O. Box 418, Cotonou, Benin.; Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2020 Oct 19; Vol. 13 (1), pp. 485. Date of Electronic Publication: 2020 Oct 19.
DOI: 10.1186/s13104-020-05332-7
Abstrakt: Objective: Rotavirus remains the main causative agent of gastroenteritis in young children in countries that have not yet introduced the vaccine. In Benin, rotavirus vaccine was introduced late December 2019 into the EPI. This study aims to provide pre-vaccination era rotavirus genotyping data in Benin. These data can supplement data from the surveillance system of Ministry of Health of Benin which is supported by the World Health Organization (WHO).
Results: Of the 420 diarrheal stool samples, actively collected in southern Benin from July 2016 through November 2018 from children under 5 years old and suffering from gastroenteritis, 167 (39.8%) samples were rotavirus EIA positive. 186 (44.3%) samples contained amplifiable rotavirus RNA detected by qRT-PCR method and were genotyped using one-step RT-PCR multiplex genotyping method. G1P[8] represents the predominant genotype (32%) followed by the G2P[4] (26%), G3P[6] (16%), G12P[8] (13%) and mixed G and P types (1%). Four samples (2%) could not be assigned both G and P type specificity.
Databáze: MEDLINE
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