Detecting and correlating bacterial populations to visual color change of polydiacetylene-coated filters.

Autor: Zhang Y; Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, USA., Dawson PL; Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, USA. Electronic address: pdawson@clemson.edu., Hanks TW; Department of Chemistry, Furman University, Greenville, SC, USA., Northcutt JK; Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, USA., Tzeng TR; Department of Biological Sciences, Clemson University, Clemson, SC, USA., Pennington WT; Department of Chemistry, Clemson University, Clemson, SC, USA.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2021 Jan 01; Vol. 221, pp. 121482. Date of Electronic Publication: 2020 Aug 05.
DOI: 10.1016/j.talanta.2020.121482
Abstrakt: Membrane filters were coated with 10,12-pentacosadiynoic acid (PCDA) then polymerized on the filter for rapid bacterial detection and quantification. The polymerized PCDA (pPDCA)-coated filter changed color in response to Salmonella Typhimurium and Escherichia coli but not to Listeria innocua. The time required for color change of pPCDA-coated filters was determined by a visual panel. A simple linear regression model was generated to fit the observed data and was validated with goodness of fit analysis and residual analysis. The pPCDA-filter method estimated Salmonella Typhimurium populations of 8 to 3 log CFU ml -1 within 1.5-7.5 h, respectively.
(Copyright © 2020. Published by Elsevier B.V.)
Databáze: MEDLINE
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