Nuclear and kinetoplast DNA analyses reveal genetically complex Leishmania strains with hybrid and mito-nuclear discordance in Peru.

Autor: Tabbabi A; Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan., Cáceres AG; Sección de Entomología, Instituto de Medicina Tropical 'Daniel A. Carrión' y Departamento Académico de Microbiología Médica, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Perú.; Laboratorio de Entomología, Instituto Nacional de Salud, Lima, Perú., Bustamante Chauca TP; Área de Investigación en Salud-Evaluación de las Intervenciones Sanitarias y Vigilancia de las Enfermedades Metaxénicas y Zoonoticas, Dirección Regional de Salud Amazonas, Perú., Seki C; Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan., Choochartpong Y; Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand., Mizushima D; Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan., Yamamoto DS; Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan., Hashiguchi Y; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan., Kato H; Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2020 Oct 19; Vol. 14 (10), pp. e0008797. Date of Electronic Publication: 2020 Oct 19 (Print Publication: 2020).
DOI: 10.1371/journal.pntd.0008797
Abstrakt: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mannose phosphate isomerase (mpi) gene was applied to 134 skin samples collected from patients with cutaneous leishmaniasis (CL) in Peru for identification of the infecting parasite at the species level, and the results were compared with those of cytochrome b (cyt b) gene sequencing obtained in previous studies. Although most results (121/134) including 4 hybrids of Leishmania (Viannia) braziliensis and L. (V.) peruviana corresponded to those obtained in the previous study, PCR-RFLP analyses revealed the distribution of putative hybrid strains between L. (V.) peruviana and L. (V.) lainsoni in two samples, which has never been reported. Moreover, parasite strains showing discordance between kinetoplast and nuclear genes (kDNA and nDNA), so-called mito-nuclear discordance, were identified in 11 samples. Of these, six strains had the kDNAs of L. (V.) braziliensis or L. (V.) peruviana and nDNAs of L. (V.) guyanensis, and three strains had the kDNAs of L. (V.) shawi and nDNAs of L. (V.) braziliensis. The rest were identified as mito-nuclear discordance strains having kDNAs of L. (V.) braziliensis or L. (V.) peruviana and nDNAs of L. (V.) lainsoni, and kDNAs of L. (V.) lainsoni and nDNAs of L. (V.) braziliensis. The results demonstrate that Leishmania strains in Peru are genetically more complex than previously considered.
Competing Interests: No authors have competing interests
Databáze: MEDLINE
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