Diverse Macrophage Populations Contribute to the Inflammatory Microenvironment in Premalignant Lesions During Localized Invasion.

Autor:	Ibrahim AM; Department of Biochemistry and Molecular Biology, Tulane School of Medicine, New Orleans, LA, United States.; Tulane Cancer Center, Louisiana Cancer Research Consortium, New Orleans, LA, United States.; Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt., Moss MA; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States., Gray Z; Department of Biochemistry and Molecular Biology, Tulane School of Medicine, New Orleans, LA, United States.; Tulane Cancer Center, Louisiana Cancer Research Consortium, New Orleans, LA, United States., Rojo MD; Department of Biochemistry and Molecular Biology, Tulane School of Medicine, New Orleans, LA, United States.; Tulane Cancer Center, Louisiana Cancer Research Consortium, New Orleans, LA, United States., Burke CM; Department of Biochemistry and Molecular Biology, Tulane School of Medicine, New Orleans, LA, United States.; Tulane Cancer Center, Louisiana Cancer Research Consortium, New Orleans, LA, United States., Schwertfeger KL; Department of Laboratory Medicine and Pathology, Masonic Cancer Center, and Center for Immunology, University of Minnesota, Minneapolis, MN, United States., Dos Santos CO; Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, NY, United States., Machado HL; Department of Biochemistry and Molecular Biology, Tulane School of Medicine, New Orleans, LA, United States.; Tulane Cancer Center, Louisiana Cancer Research Consortium, New Orleans, LA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in oncology [Front Oncol] 2020 Sep 24; Vol. 10, pp. 569985. Date of Electronic Publication: 2020 Sep 24 (Print Publication: 2020).
DOI:	10.3389/fonc.2020.569985
Abstrakt:	Myeloid cell heterogeneity remains poorly studied in breast cancer, and particularly in premalignancy. Here, we used single cell RNA sequencing to characterize macrophage diversity in mouse pre-invasive lesions as compared to lesions undergoing localized invasion. Several subpopulations of macrophages with transcriptionally distinct profiles were identified, two of which resembled macrophages in the steady state. While all subpopulations expressed tumor-promoting genes, many of the populations expressed pro-inflammatory genes, differing from reports in tumor-associated macrophages. Gene profiles of the myeloid cells were similar between early and late stages of premalignancy, although expansion of some subpopulations occurred. These results unravel macrophage heterogeneity in early progression and may provide insight into early intervention strategies that target macrophages. (Copyright © 2020 Ibrahim, Moss, Gray, Rojo, Burke, Schwertfeger, dos Santos and Machado.)
Databáze:	MEDLINE
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