Metagenomics Reveals Diet-Specific Specialization of Bacterial Communities in Fungus Gardens of Grass- and Dicot-Cutter Ants.

Autor:	Khadempour L; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.; Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, United States.; Department of Earth and Environmental Sciences, Rutgers University, Newark, Newark, NJ, United States., Fan H; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.; Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Yunnan, China., Keefover-Ring K; Departments of Botany and Geography, University of Wisconsin-Madison, Madison, WI, United States., Carlos-Shanley C; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.; Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, United States.; Department of Biology, Texas State University, San Marcos, TX, United States., Nagamoto NS; Department of Plant Protection, São Paulo State University, Botucatu, Brazil., Dam MA; Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, United States., Pupo MT; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Currie CR; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.; Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2020 Sep 24; Vol. 11, pp. 570770. Date of Electronic Publication: 2020 Sep 24 (Print Publication: 2020).
DOI:	10.3389/fmicb.2020.570770
Abstrakt:	Leaf-cutter ants in the genus Atta are dominant herbivores in the Neotropics. While most species of Atta cut dicots to incorporate into their fungus gardens, some species specialize on grasses. Here we examine the bacterial community associated with the fungus gardens of grass- and dicot-cutter ants to examine how changes in substrate input affect the bacterial community. We sequenced the metagenomes of 12 Atta fungus gardens, across four species of ants, with a total of 5.316 Gbp of sequence data. We show significant differences in the fungus garden bacterial community composition between dicot- and grass-cutter ants, with grass-cutter ants having lower diversity. Reflecting this difference in community composition, the bacterial functional profiles between the fungus gardens are significantly different. Specifically, grass-cutter ant fungus garden metagenomes are particularly enriched for genes responsible for amino acid, siderophore, and terpenoid biosynthesis while dicot-cutter ant fungus gardens metagenomes are enriched in genes involved in membrane transport. Differences between community composition and functional capacity of the bacteria in the two types of fungus gardens reflect differences in the substrates that the ants incorporated. These results show that different substrate inputs matter for fungus garden bacteria and shed light on the potential role of bacteria in mediating the ants' transition to the use of a novel substrate. (Copyright © 2020 Khadempour, Fan, Keefover-Ring, Carlos-Shanley, Nagamoto, Dam, Pupo and Currie.)
Databáze:	MEDLINE
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