Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells.

Autor: Jiang L; Department of Paediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China; Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK. Electronic address: jiangliming623@163.com., Ayre WN; Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK; Cardiff Institute for Tissue Engineering and Repair (CITER), Cardiff, UK. Electronic address: ayrewn@cardiff.ac.uk., Melling GE; Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK. Electronic address: gmelling@brookes.ac.uk., Song B; Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK. Electronic address: songb3@cardiff.ac.uk., Wei X; Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK. Electronic address: weix1@cardiff.ac.uk., Sloan AJ; Department of Paediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China; Department of Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK; Cardiff Institute for Tissue Engineering and Repair (CITER), Cardiff, UK; Melbourne Dental School, University of Melbourne, Melbourne, Victoria, Australia. Electronic address: alastair.sloan@unimelb.edu.au., Chen X; Department of Paediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China. Electronic address: chenxu@cmu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2020 Dec; Vol. 103, pp. 103501. Date of Electronic Publication: 2020 Oct 14.
DOI: 10.1016/j.jdent.2020.103501
Abstrakt: Objectives: This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration.
Methods: 0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydration method. Release of TGF-β1 from the liposomes was quantified by an enzyme-linked immunosorbent assay (ELISA). The hDPSCs were treated with different concentrations of liposomal TGF-β1 and cell viability was tested using an MTT assay. "Osteodentine" differentiation capacity was assessed by RT-qPCR, ELISA and Alizarin red S staining.
Results: The ELISA results showed that liposomal TGF-β1 achieved a controlled and prolonged release over time. The MTT results demonstrated that the liposomes (100 μg/mL) were not cytotoxic to the cells. Liposomal TGF-β1 up-regulated the expression of "osteodentine" markers, RUNX-2, DMP-1 and DSPP, in hDPSCs after 7 days of treatment and resulted in the accumulation of mineralised nodules.
Conclusion: This study indicated that liposomes are an effective carrier for delivering TGF-β1 over time. Liposomal TGF-β1 promoted dentinogenesis and increased mineralisation in hDPSCs. This highlights the potential of liposomal TGF-β1 for future use in dentine-pulp regeneration.
Clinical Significance: Liposomal TGF-β1 may be used as a synergist for promoting dentine-pulp regeneration of immature permanent teeth or as a pulp capping agent for inducing reparative dentine formation.
(Crown Copyright © 2020. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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