| Autor: |
de Mendonça RP; Department of Oral Pathology, School of Dentistry, Universidade Federal do Pará, Avenida Augusto Correa, 01, Belém, Pará, 66075-110, Brazil., Balbinot KM; Department of Oral Pathology, School of Dentistry, Universidade Federal do Pará, Avenida Augusto Correa, 01, Belém, Pará, 66075-110, Brazil., Martins BV; Department of Oral Pathology, School of Dentistry, Universidade Federal do Pará, Avenida Augusto Correa, 01, Belém, Pará, 66075-110, Brazil., da Silva Kataoka MS; Department of Oral Pathology, School of Dentistry, Universidade Federal do Pará, Avenida Augusto Correa, 01, Belém, Pará, 66075-110, Brazil., Mesquita RA; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil., de Jesus Viana Pinheiro J; Department of Oral Pathology, School of Dentistry, Universidade Federal do Pará, Avenida Augusto Correa, 01, Belém, Pará, 66075-110, Brazil. joaopinheiro@ufpa.br., de Melo Alves Júnior S; Department of Oral Pathology, School of Dentistry, Universidade Federal do Pará, Avenida Augusto Correa, 01, Belém, Pará, 66075-110, Brazil. |
| Abstrakt: |
Ameloblastomas are epithelial odontogenic tumours that, although benign, are locally invasive and may exhibit aggressive behaviour. In the tumour microenvironment, the concentration of oxygen is reduced, which leads to intratumoral hypoxia. Under hypoxia, the crosstalk between the HIF-1α, MMP-2, VEGF, and VEGFR-2 proteins has been associated with hypoxia-induced angiogenesis, leading to tumour progression and increased invasiveness. This work showcases 24 ameloblastoma cases, 10 calcifying odontogenic cysts, and 9 dental follicles, used to investigate the expression of these proteins by immunohistochemistry. The anti-HIF-1α, anti-MMP-2, anti-VEGF, and anti-VEGFR-2 primary antibodies are used in this work. The results have been expressed by the mean grey value after immunostaining in images acquired with an objective of 40×. The ameloblastoma samples showed higher immunoexpression of HIF-1α, MMP-2, VEGF, and VEGFR-2 when compared to the dental follicles and calcifying odontogenic cysts. Ameloblastomas show a higher degree of expression of proteins associated with intratumoral hypoxia and proangiogenic proteins, which indicates the possible role of these proteins in the biological behaviour of this tumour. |
