Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage.

Autor:	Lee H; Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA., Wiegand DJ; Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA., Griswold K; Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA.; MIT Media Lab, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Charles Stark Draper Laboratory, Cambridge, MA, 02139, USA., Punthambaker S; Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA., Chun H; Department of Biomedical Engineering, Korea University, 466 Hana Science Hall, 145 Anamro, Seongbukgu, 02841, Seoul, South Korea., Kohman RE; Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA. richie.kohman@wyss.harvard.edu.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA. richie.kohman@wyss.harvard.edu., Church GM; Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA. gchurch@genetics.med.harvard.edu.; Wyss Institute for Biologically Inspired Engineering, Boston, MA, 02115, USA. gchurch@genetics.med.harvard.edu.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2020 Oct 16; Vol. 11 (1), pp. 5246. Date of Electronic Publication: 2020 Oct 16.
DOI:	10.1038/s41467-020-18681-5
Abstrakt:	New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing DNA is stifled by the continued reliance on chemical synthesis methods. The enzymatic synthesis of DNA is a promising alternative, but thus far has not been well demonstrated in a parallelized manner. Here, we report a multiplexed enzymatic DNA synthesis method using maskless photolithography. Rapid uncaging of Co 2+ ions by patterned UV light activates Terminal deoxynucleotidyl Transferase (TdT) for spatially-selective synthesis on an array surface. Spontaneous quenching of reactions by the diffusion of excess caging molecules confines synthesis to light patterns and controls the extension length. We show that our multiplexed synthesis method can be used to store digital data by encoding 12 unique DNA oligonucleotide sequences with video game music, which is equivalent to 84 trits or 110 bits of data.
Databáze:	MEDLINE
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