Candida parapsilosis Infection: A Multilocus Microsatellite Genotyping-Based Survey Demonstrating an Outbreak in Hospitalized Patients.

Autor:	Calà C; Department of Science for Health Promotion and Mother to Child Care 'G. D'Alessandro' - University of Palermo, Palermo., Fontana I; Institute of Biosciences and Bioresources (IBBR), National Research Council of Italy (CNR), Naples., Di Carlo P; Department of Science for Health Promotion and Mother to Child Care 'G. D'Alessandro' - University of Palermo, Palermo., Mascarella C; Department of Science for Health Promotion and Mother to Child Care 'G. D'Alessandro' - University of Palermo, Palermo., Fasciana T; Department of Science for Health Promotion and Mother to Child Care 'G. D'Alessandro' - University of Palermo, Palermo., Reale S; Experimental Zooprophylactic Institute of Sicily A. Mirri, Palermo, Italy., Sergi C; Department of Science for Health Promotion and Mother to Child Care 'G. D'Alessandro' - University of Palermo, Palermo sergi@ualberta.ca., Giammanco A; Department of Laboratory Medicine and Pathology, University of Alberta.; Stollery Children's Hospital, Edmonton, AB, Canada.
Jazyk:	angličtina
Zdroj:	Annals of clinical and laboratory science [Ann Clin Lab Sci] 2020 Sep; Vol. 50 (5), pp. 657-664.
Abstrakt:	Microsatellite analysis identifies specific genotypes and the genetic relationship between strains. Our objective was to analyze the genotypes of C. parapsilosis strains isolated on different wards of a Tertiary-Referral University Center. We evaluated 70 C. parapsilosis strains in total, isolated from samples of patients admitted to five different wards over two years (January 2015-December 2016). Eight microsatellite markers were selected, and two multiplex PCR assays were set up for microsatellite analysis. The 70 strains, examined at eight microsatellite loci, showed 46 different multilocus genotypes profiles. A total of 74 alleles were detected, with an average of 9.25 alleles per locus. The most variable loci were CP6 and CP4, with 20 and 15 alleles, respectively. Four clusters were detected in four out of five wards. A significant cluster that involved 16 patients in the General Surgery department was also found in two patients who had been transferred to the General Medicine ward. Two multiplex PCRs allowed us to minimize costs, define genotypes and study the isolates' genetic diversity with extreme accuracy, demonstrating the high discriminative power of the microsatellite markers. Molecular epidemiology constitutes an appropriate tool for evaluating horizontal transmission of C. parapsilosis in different clinical settings. Microsatellite genotyping and the utilization of Bruvo's genetic distance are suitable for detecting and appraising nosocomial fungal infections. (© 2020 by the Association of Clinical Scientists, Inc.)
Databáze:	MEDLINE
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