| Autor: |
Sutton G; LABÉO Frank Duncombe, 14280 Saint-Contest, France.; BIOTARGEN, Normandie Univ, UNICAEN, 14000 Caen, France., Thieulent C; LABÉO Frank Duncombe, 14280 Saint-Contest, France.; BIOTARGEN, Normandie Univ, UNICAEN, 14000 Caen, France., Fortier C; LABÉO Frank Duncombe, 14280 Saint-Contest, France.; BIOTARGEN, Normandie Univ, UNICAEN, 14000 Caen, France.; ImpedanCELL, Normandie Univ, UNICAEN, 14280 Saint-Contest, France., Hue ES; LABÉO Frank Duncombe, 14280 Saint-Contest, France.; BIOTARGEN, Normandie Univ, UNICAEN, 14000 Caen, France.; ImpedanCELL, Normandie Univ, UNICAEN, 14280 Saint-Contest, France., Marcillaud-Pitel C; RESPE, 14280 Saint-Contest, France., Pléau A; INRAE, UE-1277 Plateforme d'Infectiologie Expérimentale (PFIE), Centre de Recherche Val de Loire, 37380 Nouzilly, France., Deslis A; INRAE, UE-1277 Plateforme d'Infectiologie Expérimentale (PFIE), Centre de Recherche Val de Loire, 37380 Nouzilly, France., Guitton E; INRAE, UE-1277 Plateforme d'Infectiologie Expérimentale (PFIE), Centre de Recherche Val de Loire, 37380 Nouzilly, France., Paillot R; LABÉO Frank Duncombe, 14280 Saint-Contest, France.; BIOTARGEN, Normandie Univ, UNICAEN, 14000 Caen, France.; School of Equine and Veterinary Physiotherapy, Writtle University College, Lordship Road, Writtle, Chelmsford CM1 3RR, UK., Pronost S; LABÉO Frank Duncombe, 14280 Saint-Contest, France.; BIOTARGEN, Normandie Univ, UNICAEN, 14000 Caen, France.; ImpedanCELL, Normandie Univ, UNICAEN, 14280 Saint-Contest, France. |
| Abstrakt: |
Equid herpesvirus 1 is one of the most common viral pathogens in the horse population and is associated with respiratory disease, abortion and still-birth, neonatal death and neurological disease. A single point mutation in the DNA polymerase gene (ORF30: A2254G, N752D) has been widely associated with neuropathogenicity of strains, although this association has not been exclusive. This study describes the fortuitous isolation of a strain carrying a new genotype C 2254 (H 752 ) from an outbreak in France that lasted several weeks in 2018 and involved 82 horses, two of which showed neurological signs of disease. The strain was characterised as U L clade 10 using the equid herpesvirus 1 (EHV-1) multi-locus sequence typing (MLST) classification but has not been identified or isolated since 2018. The retrospective screening of EHV-1 strains collected between 2016 and 2018 did not reveal the presence of the C 2254 mutation. When cultured in vitro, the C 2254 EHV-1 strain induced a typical EHV-1 syncytium and cytopathic effect but no significant difference was observed when compared with A 2254 and G 2254 EHV-1 strains. An experimental infection was carried out on four Welsh mountain ponies to confirm the infectious nature of the C 2254 strain. A rapid onset of marked respiratory disease lasting at least 2 weeks, with significant virus shedding and cell-associated viraemia, was observed. Finally, an in vitro antiviral assay using impedance measurement and viral load quantification was performed with three antiviral molecules (ganciclovir (GCV), aciclovir (ACV) and aphidicolin (APD)) on the newly isolated C 2254 strain and two other A/G 2254 field strains. The three strains showed similar sensitivity to ganciclovir and aphidicolin but both C 2254 and A 2254 strains were more sensitive to aciclovir than the G 2254 strain, based on viral load measurement. |
