Genetic, Morphological and Antigenic Relationships between Mesonivirus Isolates from Australian Mosquitoes and Evidence for Their Horizontal Transmission.

Autor: Newton ND; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia., Colmant AMG; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia., O'Brien CA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia., Ledger E; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia., Paramitha D; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia., Bielefeldt-Ohmann H; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia.; Australian Infectious Diseases Research Centre, University of Queensland, St Lucia, 4072 Queensland, Australia.; School of Veterinary Science, University of Queensland Gatton Campus, 4343 Queensland, Australia., Watterson D; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia.; Australian Infectious Diseases Research Centre, University of Queensland, St Lucia, 4072 Queensland, Australia., McLean BJ; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia., Hall-Mendelin S; Public Health Virology, Queensland Health Forensic and Scientific Services, 4108 Queensland, Australia., Warrilow D; Public Health Virology, Queensland Health Forensic and Scientific Services, 4108 Queensland, Australia., van den Hurk AF; Public Health Virology, Queensland Health Forensic and Scientific Services, 4108 Queensland, Australia., Liu W; Australian Defence Force Malaria and Infectious Disease Institute, Gallipoli Barracks, Enoggera, 4051 Queensland, Australia., Hoare C; Australian Defence Force Malaria and Infectious Disease Institute, Gallipoli Barracks, Enoggera, 4051 Queensland, Australia., Kizu JR; Australian Defence Force Malaria and Infectious Disease Institute, Gallipoli Barracks, Enoggera, 4051 Queensland, Australia., Gauci PJ; Defence Science & Technology Group, Fisherman's Bend, 3207 Victoria, Australia., Haniotis J; NSW Health Pathology-ICPMR Westmead, Medical Entomology, Westmead Hospital, Westmead, 2145 New South Wales, Australia., Doggett SL; NSW Health Pathology-ICPMR Westmead, Medical Entomology, Westmead Hospital, Westmead, 2145 New South Wales, Australia., Shaban B; Australian Genome Research Facility Ltd., Parkville, 3050 Victoria, Australia., Johansen CA; School of Pathology and Laboratory Medicine, WA, 6009 Nedlands, Western Australia, Australia.; PathWest Laboratory Medicine WA, 6009 Nedlands, Western Australia, Australia., Hall RA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia.; Australian Infectious Diseases Research Centre, University of Queensland, St Lucia, 4072 Queensland, Australia., Hobson-Peters J; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2020 Oct 13; Vol. 12 (10). Date of Electronic Publication: 2020 Oct 13.
DOI: 10.3390/v12101159
Abstrakt: The Mesoniviridae are a newly assigned family of viruses in the order Nidovirales . Unlike other nidoviruses, which include the Coronaviridae , mesoniviruses are restricted to mosquito hosts and do not infect vertebrate cells. To date there is little information on the morphological and antigenic characteristics of this new group of viruses and a dearth of mesonivirus-specific research tools. In this study we determined the genetic relationships of recent Australian isolates of Alphamesonivirus 4 (Casuarina virus-CASV) and Alphamesonivirus 1 (Nam Dinh virus-NDiV), obtained from multiple mosquito species. Australian isolates of NDiV showed high-level similarity to the prototype NDiV isolate from Vietnam (99% nucleotide (nt) and amino acid (aa) identity). Isolates of CASV from Central Queensland were genetically very similar to the prototype virus from Darwin (95-96% nt and 91-92% aa identity). Electron microscopy studies demonstrated that virion diameter (≈80 nm) and spike length (≈10 nm) were similar for both viruses. Monoclonal antibodies specific to CASV and NDiV revealed a close antigenic relationship between the two viruses with 13/34 mAbs recognising both viruses. We also detected NDiV RNA on honey-soaked nucleic acid preservation cards fed on by wild mosquitoes supporting a possible mechanism of horizontal transmission between insects in nature.
Databáze: MEDLINE