Biological activities of a new crotamine-like peptide from Crotalus oreganus helleri on C2C12 and CHO cell lines, and ultrastructural changes on motor endplate and striated muscle.

Autor: Salazar E; National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX, USA., Rodriguez-Acosta A; Laboratorio de Inmunoquímica y Ultraestructura, Instituto Anatómico, Universidad Central de Venezuela, Caracas, Venezuela., Lucena S; National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX, USA., Gonzalez R; Centro de Microscopía Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela., McLarty MC; National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX, USA., Sanchez O; National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX, USA., Suntravat M; National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX, USA., Garcia E; Centro de Microscopía Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela., Finol HJ; Centro de Microscopía Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela., Giron ME; Laboratorio de Inmunoquímica y Ultraestructura, Instituto Anatómico, Universidad Central de Venezuela, Caracas, Venezuela., Fernandez I; Laboratorio de Inmunoquímica y Ultraestructura, Instituto Anatómico, Universidad Central de Venezuela, Caracas, Venezuela., Deba F; Texas A&M Rangel College of Pharmacy, Kingsville, TX, USA., Bessac BF; Texas A&M Rangel College of Pharmacy, Kingsville, TX, USA; Jerry H. Hodge School of Pharmacy, Texas Tech University HSC, Amarillo, TX, USA., Sánchez EE; National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX, USA; Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX, USA. Electronic address: elda.sanchez@tamuk.edu.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2020 Dec; Vol. 188, pp. 95-107. Date of Electronic Publication: 2020 Oct 13.
DOI: 10.1016/j.toxicon.2020.10.010
Abstrakt: Crotamine and crotamine-like peptides are non-enzymatic polypeptides, belonging to the family of myotoxins, which are found in high concentration in the venom of the Crotalus genus. Helleramine was isolated and purified from the venom of the Southern Pacific rattlesnake, Crotalus oreganus helleri. This peptide had a similar, but unique, identity to crotamine and crotamine-like proteins isolated from other rattlesnakes species. The variability of crotamine-like protein amino acid sequences may allow different toxic effects on biological targets or optimize the action against the same target of different prey. Helleramine was capable of increasing intracellular Ca 2+ in Chinese Hamster Ovary (CHO) cell line. It inhibited cell migration as well as cell viability (IC 50  = 11.44 μM) of C2C12, immortalized skeletal myoblasts, in a concentration dependent manner, and promoted early apoptosis and cell death under our experimental conditions. Skeletal muscle harvested from mice 24 h after helleramine injection showed contracted myofibrils and profound vacuolization that enlarged the subsarcolemmal space, along with loss of plasmatic and basal membrane integrity. The effects of helleramine provide further insights and evidence of myotoxic activities of crotamine-like peptides and their possible role in crotalid envenomings.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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