Screening and Cellular Characterization of Genetically Encoded Voltage Indicators Based on Near-Infrared Fluorescent Proteins.

Autor:	Monakhov MV; Institute for Systems Genomics, Stem Cell Institute, Department of Neuroscience, UConn Health, Farmington, Connecticut 06030, United States.; Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York 10461, United States., Matlashov ME; Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York 10461, United States., Colavita M; Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, U.K., Song C; Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, U.K., Shcherbakova DM; Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York 10461, United States., Antic SD; Institute for Systems Genomics, Stem Cell Institute, Department of Neuroscience, UConn Health, Farmington, Connecticut 06030, United States., Verkhusha VV; Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York 10461, United States., Knöpfel T; Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, U.K.
Jazyk:	angličtina
Zdroj:	ACS chemical neuroscience [ACS Chem Neurosci] 2020 Nov 04; Vol. 11 (21), pp. 3523-3531. Date of Electronic Publication: 2020 Oct 16.
DOI:	10.1021/acschemneuro.0c00046
Abstrakt:	We developed genetically encoded voltage indicators using a transmembrane voltage-sensing domain and bright near-infrared fluorescent proteins derived from bacterial phytochromes. These new voltage indicators are excited by 640 nm light and emission is measured at 670 nm, allowing imaging in the near-infrared tissue transparency window. The spectral properties of our new indicators permit seamless voltage imaging with simultaneous blue-green light optogenetic actuator activation as well as simultaneous voltage-calcium imaging when paired with green calcium indicators. Iterative optimizations led to a fluorescent probe, here termed nirButterfly, which reliably reports neuronal activities including subthreshold membrane potential depolarization and hyperpolarization as well as spontaneous spiking or electrically- and optogenetically evoked action potentials. This enables largely improved all-optical causal interrogations of physiology.
Databáze:	MEDLINE
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