Comparison of the effect on proliferation of osteoblasts MC3T3 - E1 between the concentrated growth factor extract and the platelet - rich fibrin extract.

Autor:	Wei Z; Departmentof Oral and Maxillofacial Surgery, Haikou Hospital Affiliated to Xiangya Medical School, Central South University, Haikou 570208. 359322794@qq.com., Liu S; Department of Dental Implantology, Haikou Hospital Affiliated to Xiangya Medical School, Central South University, Haikou 570208, China., Chen Z; Department of Dental Implantology, Haikou Hospital Affiliated to Xiangya Medical School, Central South University, Haikou 570208, China., Huang X; Departmentof Oral and Maxillofacial Surgery, Haikou Hospital Affiliated to Xiangya Medical School, Central South University, Haikou 570208. hnsun3@126.com.
Jazyk:	English; Chinese
Zdroj:	Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2020 Aug 28; Vol. 45 (8), pp. 901-908.
DOI:	10.11817/j.issn.1672-7347.2020.190037
Abstrakt:	Objectives: To compare the effect on proliferation of osteoblasts MC3T3-E1 between the concentrated growth factor extract (CGFe) and the platelet-rich fibrin extract (PRFe). Methods: CGFe and PRFe were prepared. MC3T3-E1 was cultured in DMEM medium containing CGFe (10%, 20%, or 30%) and PRFe (10%, 20%, or 30%). The proliferation of MC3T3-E1 was detected by MTT assay at Day 1, 3, 5, and 7. ALP activity was detected by alkaline phosphatase (ALP) staining at Day 1, 3, 5, and 7, and mRNA expressions of Runt-related transcription factor 2 (Runx2) and Osterix (Osx) were detected by quantitative RT-PCR (RT-qPCR) at Day 3 and 7. Results: Compared with the control group, CGFe and PRFe promoted the proliferation of MC3T3-E1 at Day 1, 3, 5, and 7 (all P <0.05). Except for the first day, the proliferation activity in the CGFe group was higher than that in the PRFe group (all P <0.05). At Day 1, 3, 5, and 7, compared with the control group, the ALP activities in the CGFe group and the PRFe group were significantly increased (all P <0.05). Except for the first day, the ALP activity in the CGFe group was higher than that in the PRFe group (all P <0.05). At Day 3 and 7, compared with the control group, the mRNA expression levels of Osx and Runx2 in the CGFe group and the PRFe group were significantly increased (all P <0.05); compared with PRFe group, the mRNA expression level of Osx in the CGFe group was significantly higher than that in the PRFe group, and the mRNA expression level of Runx2 was significantly lower than that in the PRFe group (all P <0.05). Conclusions: CGFe could promote the proliferation of MC3T3-E1 stronger than PRFe, which might be related to the increase of ALP activity and up-regulation of Osx expression.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu