Diagnostic significance of flow cytometry scales in diagnostics of myelodysplastic syndromes.

Autor: Davydova YO; Laboratory of Immunophenotyping, National Research Center for Hematology, Moscow, Russia., Parovichnikova EN; Chemotherapy Department for Hemoblastoses, Hemopoiesis Depression and BMT, National Research Center for Hematology, Moscow, Russia., Galtseva IV; Laboratory of Immunophenotyping, National Research Center for Hematology, Moscow, Russia., Kokhno AV; Intensive High-Dose Chemotherapy Department for Hemoblastoses and Hematopoiesis Depressions, National Research Center for Hematology, Moscow, Russia., Dvirnyk VN; Centralized Diagnostic Laboratory, National Research Center for Hematology, Moscow, Russia., Kovrigina AM; Department of Pathology, National Research Center for Hematology, Moscow, Russia., Obukhova TN; Karyology Laboratory, National Research Center for Hematology, Moscow, Russia., Kapranov NM; Laboratory of Immunophenotyping, National Research Center for Hematology, Moscow, Russia., Nikiforova KA; Laboratory of Immunophenotyping, National Research Center for Hematology, Moscow, Russia., Glinkina SA; Department of Pathology, National Research Center for Hematology, Moscow, Russia., Troitskaya VV; Intensive High-Dose Chemotherapy Department for Hemoblastoses and Hematopoiesis Depressions, National Research Center for Hematology, Moscow, Russia., Mikhailova EA; Intensive High-Dose Chemotherapy Department for Hemoblastoses and Hematopoiesis Depressions, National Research Center for Hematology, Moscow, Russia., Fidarova ZT; Intensive High-Dose Chemotherapy Department for Hemoblastoses and Hematopoiesis Depressions, National Research Center for Hematology, Moscow, Russia., Moiseeva TN; Department of Hematology Advisory, National Research Center for Hematology, Moscow, Russia., Lukina EA; Department of Orphan Diseases, National Research Center for Hematology, Moscow, Russia., Tsvetaeva NV; Department of Orphan Diseases, National Research Center for Hematology, Moscow, Russia., Nikulina OF; Department of Orphan Diseases, National Research Center for Hematology, Moscow, Russia., Kuzmina LA; Department of Intensive High-Dose Chemotherapy and BMT, National Research Center for Hematology, Moscow, Russia., Savchenko VG; Head of National Research Center for Hematology, Moscow, Russia.
Jazyk: angličtina
Zdroj: Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2021 May; Vol. 100 (3), pp. 312-321. Date of Electronic Publication: 2020 Oct 14.
DOI: 10.1002/cyto.b.21965
Abstrakt: Background: Myelodysplastic syndromes (MDS) can present a challenge for clinicians. Multicolor flow cytometry (MFC) can aid in establishing a diagnosis. The aim of this study was to determine the optimal MFC approach for MDS.
Methods: The study included 102 MDS (39 low-grade MDS), 83 cytopenic patients without myeloid neoplastic disorders (control group), and 35 healthy donors. Bone marrow was analyzed using a six-color MFC. Analysis was conducted according to the "Ogata score," "Wells score," and the integrated flow cytometry (iFC) score.
Results: The respective sensitivity and specificity values were 77.5% and 90.4% for the Ogata score, 79.4% and 81.9% for the Wells score, and 87.3% and 87.6% for the iFC score. Specificity was not 100% due to deviations of MFC parameters in the control group. Patients with paroxysmal nocturnal hemoglobinuria (PNH) had higher levels of CD34 + CD7 + myeloid cells than donors. Aplastic anemia and PNH were characterized by a high proportion of CD56 + cells among CD34 + precursors and neutrophils. The proportion of MDS-related features increased with the progression of MDS. The highest number of CD34 + blasts was found in MDS with excess blasts. MDS with isolated del(5q) was characterized by a high proportion of CD34 + CD7 + cells and low granularity of neutrophils. In 39 low-grade MDS, the sensitivities were 53.8%, 61.5%, and 71.8% for Ogata score, Wells score, and iFC, respectively.
Conclusion: The results support iFC as a useful diagnostic tool in MDS.
(© 2020 International Clinical Cytometry Society.)
Databáze: MEDLINE
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