Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications.

Autor: Krasitskaya VV; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia., Bashmakova EE; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia., Frank LA; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia.; School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2020 Oct 10; Vol. 21 (20). Date of Electronic Publication: 2020 Oct 10.
DOI: 10.3390/ijms21207465
Abstrakt: : The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate-coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization-Ca 2+ -regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme-substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.
Competing Interests: The authors declare no conflict of interest. The funder had no role in the study design; collection, analysis, and interpretation of the data; or the decision to prepare or to publish the manuscript.
Databáze: MEDLINE
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