Electrospun Nanodiamond-Silk Fibroin Membranes: A Multifunctional Platform for Biosensing and Wound-Healing Applications.

Autor:	Khalid A; School of Science, RMIT University, Melbourne, Victoria 3001, Australia.; Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Bai D; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Abraham AN; School of Science, RMIT University, Melbourne, Victoria 3001, Australia.; Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Jadhav A; School of Fashion and Textiles, RMIT University, Brunswick, Victoria 3056, Australia., Linklater D; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Matusica A; School of Computer Science, Engineering and Mathematics, Flinders University, Clovelly Park, South Australia 5042, Australia., Nguyen D; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Murdoch BJ; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Zakhartchouk N; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Dekiwadia C; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Reineck P; School of Science, RMIT University, Melbourne, Victoria 3001, Australia.; Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Simpson D; School of Physics, University of Melbourne, Parkville, Victoria 3052, Australia., Vidanapathirana AK; Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), Adelaide Medical School, Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, South Australia 5005, Australia.; Vascular Research Centre, Lifelong Health, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, South Australia 5001, Australia., Houshyar S; School of Engineering, RMIT University, Melbourne, Victoria 3001, Australia., Bursill CA; Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), Adelaide Medical School, Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, South Australia 5005, Australia.; Vascular Research Centre, Lifelong Health, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, South Australia 5001, Australia., Ivanova EP; School of Science, RMIT University, Melbourne, Victoria 3001, Australia., Gibson BC; School of Science, RMIT University, Melbourne, Victoria 3001, Australia.; Australian Research Council (ARC) Centre of Excellence for Nanoscale BioPhotonics (CNBP), School of Science, RMIT University, Melbourne, Victoria 3001, Australia.
Jazyk:	angličtina
Zdroj:	ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2020 Oct 28; Vol. 12 (43), pp. 48408-48419. Date of Electronic Publication: 2020 Oct 13.
DOI:	10.1021/acsami.0c15612
Abstrakt:	Next generation wound care technology capable of diagnosing wound parameters, promoting healthy cell growth, and reducing pathogenic infections noninvasively would provide patients with an improved standard of care and accelerated wound repair. Temperature is one of the indicating biomarkers specific to chronic wounds. This work reports a hybrid, multifunctional optical material platform-nanodiamond (ND)-silk membranes as biopolymer dressings capable of temperature sensing and promoting wound healing. The hybrid structure was fabricated through electrospinning, and 3D submicron fibrous membranes with high porosity were formed. Silk fibers are capable of compensating for the lack of an extracellular matrix at the wound site, supporting the wound-healing process. Negatively charged nitrogen vacancy (NV - ) color centers in NDs exhibit optically detected magnetic resonance (ODMR) and act as nanoscale thermometers. This can be exploited to sense temperature variations associated with the presence of infection or inflammation in a wound, without physically removing the dressing. Our results show that the presence of NDs in the hybrid ND-silk membranes improves the thermal stability of silk fibers. NV - color centers in NDs embedded in silk fibers exhibit well-retained fluorescence and ODMR. Using the NV - centers as fluorescent nanoscale thermometers, we achieved temperature sensing in 25-50 °C, including the biologically relevant temperature window, for cell-grown ND-silk membranes. An enhancement (∼1.5× on average) in the temperature sensitivity of the NV - centers was observed for the hybrid materials. The hybrid membranes were further tested in vivo in a murine wound-healing model and demonstrated biocompatibility and equivalent wound closure rates as the control wounds. Additionally, the hybrid ND-silk membranes exhibited selective antifouling and biocidal propensity toward Gram-negative Pseudomonas aeruginosa and Escherichia coli , while no effect was observed on Gram-positive Staphylococcus aureus .
Databáze:	MEDLINE
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