Brain profiling of endogenous Neuropeptide Y (NPY) in distinct reproductive phases of adult male Microhyla ornata.

Autor: Hadawale KN; Department of Zoology, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411 007, India., Sagarkar S; Department of Zoology, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411 007, India. Electronic address: sneha.sagarkar@unipune.ac.in., Bhargava SY; Department of Zoology, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411 007, India. Electronic address: shobha@unipune.ac.in.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2021 Jan 01; Vol. 740, pp. 135409. Date of Electronic Publication: 2020 Oct 09.
DOI: 10.1016/j.neulet.2020.135409
Abstrakt: Neuropeptide Y(NPY) is known to play a pivotal role in various physiological functions including appetite and reproduction. While studies in mammals, fishes and reptiles suggest a temporal and evolutionary conserved role of NPY, the information in amphibian is scanty. We have investigated the reproductive phase related variations of NPY in the brain of Microhyla ornata (M. ornata), using immunohistochemistry and reverse transcription quantitative PCR (RT-qPCR). The highest expression of NPY peptide was observed in the preoptic area (Poa), nucleus infundibularis ventralis (NIV) and nucleus reticularis isthmi (NRIS) of M. ornata in breeding season compared to pre-breeding as well as post-breeding season. In parallel, highest mRNA levels of NPY were also observed in the breeding season in the middle region of brain that includes hypothalamus of M. ornata. Variation in the levels of NPY peptide and mRNA levels in the brain of M. ornata point towards seasonal control of appetite and reproduction.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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