Landscape of coordinated immune responses to H1N1 challenge in humans.

Autor: Rahil Z; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Leylek R; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Schürch CM; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Chen H; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Bjornson-Hooper Z; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Christensen SR; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Gherardini PF; Parker Institute for Cancer Immunotherapy, San Francisco, California, USA., Bhate SS; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.; Department of Bioengineering, Stanford University, Stanford, California, USA., Spitzer MH; Department of Microbiology and Immunology and., Fragiadakis GK; UCSF Data Science CoLab and UCSF Department of Medicine, UCSF, San Francisco, California, USA., Mukherjee N; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Kim N; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Jiang S; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Yo J; ARK Clinical Research, Long Beach, California, USA., Gaudilliere B; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, Stanford, California, USA., Affrime M; WCCT Global, Cypress, California, USA., Bock B; WCCT Global, Cypress, California, USA., Hensley SE; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Idoyaga J; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., Aghaeepour N; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, Stanford, California, USA., Kim K; ARK Clinical Research, Long Beach, California, USA., Nolan GP; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA., McIlwain DR; Department of Pathology and.; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.; WCCT Global, Cypress, California, USA.
Jazyk: angličtina
Zdroj: The Journal of clinical investigation [J Clin Invest] 2020 Nov 02; Vol. 130 (11), pp. 5800-5816.
DOI: 10.1172/JCI137265
Abstrakt: Influenza is a significant cause of morbidity and mortality worldwide. Here we show changes in the abundance and activation states of more than 50 immune cell subsets in 35 individuals over 11 time points during human A/California/2009 (H1N1) virus challenge monitored using mass cytometry along with other clinical assessments. Peak change in monocyte, B cell, and T cell subset frequencies coincided with peak virus shedding, followed by marked activation of T and NK cells. Results led to the identification of CD38 as a critical regulator of plasmacytoid dendritic cell function in response to influenza virus. Machine learning using study-derived clinical parameters and single-cell data effectively classified and predicted susceptibility to infection. The coordinated immune cell dynamics defined in this study provide a framework for identifying novel correlates of protection in the evaluation of future influenza therapeutics.
Databáze: MEDLINE