Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics.

Autor: Tahmasebi R; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo, Brazil.; Polytechnic School of University of Sao Paulo, Sao Paulo, Brazil., Luchs A; Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Sao Paulo, Brazil., Tardy K; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo, Brazil., Hefford PM; University Hospitals Plymouth NHS Trust, Plymouth, UK., Tinker RJ; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PL, UK., Eilami O; School of Medicine Social, Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran., de Padua Milagres FA; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, Brazil.; Secretary of Health of Tocantins, Tocantins, Brazil.; Institute of Biological Sciences, Federal University of Tocantins, Tocantins, Brazil., Brustulin R; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, Brazil.; Institute of Biological Sciences, Federal University of Tocantins, Tocantins, Brazil.; Secretary of Health of Tocantins, Tocantins, Brazil., Teles MDAR; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, Brazil.; Secretary of Health of Tocantins, Tocantins, Brazil., Dos Santos Morais V; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo, Brazil., Moreira CHV; Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Sao Paulo, Brazil., Buccheri R; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo, Brazil., Araújo ELL; General Coordination of Public Health Laboratories of the Strategic Articulation Department of the Health Surveillance Secretariat of the Ministry of Health (CGLAB/DAEVS/SVS-MS), Brasília, DF, Brazil., Villanova F; Institute of Biological Sciences, Federal University of Para, Para, Brazil., Deng X; Department Laboratory Medicine, University of California San Francisco, San Francisco, California, USA.; Vitalant Research Institute, San Francisco, California, USA., Sabino EC; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo, Brazil.; Polytechnic School of University of Sao Paulo, Sao Paulo, Brazil., Delwart E; Department Laboratory Medicine, University of California San Francisco, San Francisco, California, USA.; Vitalant Research Institute, San Francisco, California, USA., Leal É; Institute of Biological Sciences, Federal University of Para, Para, Brazil., Charlys da Costa A; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: The Journal of general virology [J Gen Virol] 2020 Dec; Vol. 101 (12), pp. 1280-1288. Date of Electronic Publication: 2020 Oct 12.
DOI: 10.1099/jgv.0.001500
Abstrakt: Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8 % (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9 % (55/251) of samples and mixed infections in 37.4 % (94/251), with RVA/HAdV-F being the most frequent association (21.5 %; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.
Databáze: MEDLINE