Exploration of surface glycoprotein to design multi-epitope vaccine for the prevention of Covid-19.

Autor:	Oladipo EK; Department of Microbiology, Laboratory of Molecular Biology, Immunology and Bioinformatics, Adeleke University, Ede, Osun State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Ajayi AF; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Ariyo OE; Department of Medicine, Infectious Diseases and Tropical Medicine Unit, Federal Teaching Hospital, Ido-Ekiti, Ekiti State, Nigeria., Onile SO; Biotechnology Programme, Department of Biological Sciences, Elizade University, Nigeria., Jimah EM; Department of Medical Microbiology and Parasitology, University of Ilorin, Kwara State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Ezediuno LO; Department of Microbiology and Parasitology, University of Ilorin, Kwara State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Adebayo OI; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Adebayo ET; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Odeyemi AN; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Oyeleke MO; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Oyewole MP; Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Oguntomi AS; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Akindiya OE; Microbiology Programme, Department of Biological Science, Olusegun Agagu University of Science and Technology, Okitipupa, Ondo State, Nigeria., Olamoyegun BO; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Aremu VO; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo state, Nigeria., Arowosaye AO; Department of Virology, University of Ibadan, Ibadan, Oyo State, Nigeria., Aboderin DO; Department of Medical Microbiology and Parasitology, University of Lagos, Lagos State, Nigeria., Bello HB; Department of Microbiology, Kebbi State University, Aliero, Kebbi State, Nigeria., Senbadejo TY; Department of Microbiology, Fountain University, Osogbo, Osun State, Nigeria., Awoyelu EH; Department of Natural Sciences, Precious Conerstone University, Ibadan, Oyo State, Nigeria., Oladipo AA; Department of Haematology and Blood Grouping Serology, Obafemi Awolowo Teaching Hospital Complex, Ile-Ife Wesley Guild Hospital Wing, Osun State, Nigeria., Oladipo BB; Department of Clinical Nursing, Bowen University Teaching Hospital, Ogbomoso, Oyo State, Nigeria., Ajayi LO; Department of Biochemistry, Adeleke University, Ede, Osun State, Nigeria., Majolagbe ON; Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Oyawoye OM; Department of Microbiology, Laboratory of Molecular Biology, Immunology and Bioinformatics, Adeleke University, Ede, Osun State, Nigeria., Oloke JK; Department of Natural Sciences, Precious Conerstone University, Ibadan, Oyo State, Nigeria.
Jazyk:	angličtina
Zdroj:	Informatics in medicine unlocked [Inform Med Unlocked] 2020; Vol. 21, pp. 100438. Date of Electronic Publication: 2020 Oct 04.
DOI:	10.1016/j.imu.2020.100438
Abstrakt:	Stimulation and generation of T and B cell-mediated long-term immune response are essential for the curbing of a deadly virus such as SAR-CoV-2 (Severe Acute Respiratory Corona Virus 2). Immunoinformatics approach in vaccine design takes advantage of antigenic and non-allergenic epitopes present on the spike glycoprotein of SARS-CoV-2 to elicit immune responses. T cells and B cells epitopes were predicted, and the selected residues were subjected to allergenicity, antigenicity and toxicity screening which were linked by appropriate linkers to form a multi-epitope subunit vaccine. The physiochemical properties of the vaccine construct were analyzed, and the molecular weight, molecular formula, theoretical isoelectric point value, half-life, solubility score, instability index, aliphatic index and GRAVY were predicted. The vaccine structure was constructed, refined, validated, and disulfide engineered to get the best model. Molecular binding simulation and molecular dynamics simulation were carried out to predict the stability and binding affinity of the vaccine construct with TLRs. Codon acclimatization and in silico cloning were performed to confirm the vaccine expression and potency. Results obtained indicated that this novel vaccine candidate is non-toxic, capable of initiating the immunogenic response and will not induce an allergic reaction. The highest binding energy was observed in TLR4 (Toll-like Receptor 4) (-1398.1), and the least is TLR 2 (-1479.6). The steady rise in Th (T-helper) cell population with memory development was noticed, and IFN-g (Interferon gamma) was provoked after simulation. At this point, the vaccine candidate awaits animal trial to validate its efficacy and safety for use in the prevention of the novel COVID-19 (Coronavirus Disease 2019) infections. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2020 The Author(s).)
Databáze:	MEDLINE
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