Assessing the effects of an acute exposure to worst-case concentration of Cry proteins on zebrafish using the embryotoxicity test and proteomics analysis.

Autor: Vieira L; Post-Graduation Program in Biochemistry, Department of Biochemistry and Molecular Biology, Building 907, Campus Pici, Federal University of Ceara, 60455-970, Fortaleza, Brazil., Hissa DC; Department of Biology, Building 909, Campus Pici, Federal University of Ceara, 60455-970, Fortaleza, Brazil., Souza T; Department of Toxicogenomics, GROW School for Oncology and Developmental Oncology, Maastricht University, Maastricht, the Netherlands., Gonçalves ÍFS; Post-Graduation Program in Biochemistry, Department of Biochemistry and Molecular Biology, Building 907, Campus Pici, Federal University of Ceara, 60455-970, Fortaleza, Brazil; Laboratory for Risk Assessment of Novel Technologies, Department of Molecular Biology, Federal University of Paraiba, 58051-900, João Pessoa, Brazil., Evaristo JAM; Laboratory of Proteomics, LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil., Nogueira FCS; Laboratory of Proteomics, LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil; Proteomics Unit, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil., Carvalho AFU; Post-Graduation Program in Biochemistry, Department of Biochemistry and Molecular Biology, Building 907, Campus Pici, Federal University of Ceara, 60455-970, Fortaleza, Brazil; Department of Biology, Building 909, Campus Pici, Federal University of Ceara, 60455-970, Fortaleza, Brazil., Farias D; Post-Graduation Program in Biochemistry, Department of Biochemistry and Molecular Biology, Building 907, Campus Pici, Federal University of Ceara, 60455-970, Fortaleza, Brazil; Laboratory for Risk Assessment of Novel Technologies, Department of Molecular Biology, Federal University of Paraiba, 58051-900, João Pessoa, Brazil. Electronic address: davi@dbm.ufpb.br.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2021 Feb; Vol. 264 (Pt 2), pp. 128538. Date of Electronic Publication: 2020 Oct 05.
DOI: 10.1016/j.chemosphere.2020.128538
Abstrakt: Cry1C, Cry1F and Cry1Ab are insecticidal proteins from Bacillus thuringiensis (Bt) which are expressed in transgenic crops. Given the entry of these proteins into aquatic environments, it is relevant to evaluate their impacts on aquatic organisms. In this work, we sought to evaluate the effects of Cry1C, Cry1F and Cry1Ab on zebrafish embryos and larvae of a predicted worst-case scenario concentration of these proteins (set to 1.1 mg/L). For that, we coupled a traditional toxicity approach (the zebrafish embryotoxicity test and dosage of enzymatic biomarkers) to gel free proteomics analysis. At the concentration tested, these proteins did not cause adverse effects in the zebrafish early life stages, either by verifying phenotypic endpoints of toxicity or alterations in representative enzymatic biomarkers (catalase, glutathione-S-tranferase and lactate-dehydrogenase). At the molecular level, the Cry proteins tested lead to very small changes in the proteome of zebrafish larvae. In a global way, these proteins upregulated the expression of vitellogenins. Besides that, Cry1C e Cry1F deregulated heterogeneous nuclear ribonucleoproteins (Hnrnpa0l and Hnrnpaba, respectively), implicated in mRNA processing and gene regulation. Overall, these data lead to the conclusion that Cry1C, Cry1F and Cry1Ab proteins, even at a very high concentration, have limited effects in the early stages of zebrafish life.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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