First detection and molecular characterisation of pseudocowpox virus in a cattle herd in Zambia.

Autor: Ziba MW; Department of Veterinary Services Ministry of Fisheries and Livestock, Central Veterinary Research Institute, P.O Box 33980, Lusaka, Zambia. zibamw@yahoo.com., Chitala C; Department of Veterinary Services Ministry of Fisheries and Livestock, Central Veterinary Research Institute, P.O Box 33980, Lusaka, Zambia., Settypalli TBK; Department of Nuclear Sciences and Applications, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400, Vienna, Austria., Mumba M; Department of Veterinary Services Ministry of Fisheries and Livestock, Central Veterinary Research Institute, P.O Box 33980, Lusaka, Zambia., Cattoli G; Department of Nuclear Sciences and Applications, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400, Vienna, Austria., Fandamu P; Department of Veterinary Services Ministry of Fisheries and Livestock, Central Veterinary Research Institute, P.O Box 33980, Lusaka, Zambia., Lamien CE; Department of Nuclear Sciences and Applications, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400, Vienna, Austria.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2020 Oct 09; Vol. 17 (1), pp. 152. Date of Electronic Publication: 2020 Oct 09.
DOI: 10.1186/s12985-020-01426-7
Abstrakt: Background: Pseudocowpox virus (PCPV) of the genus Parapoxvirus in the family Poxviridae causes pseudocowpox in cattle worldwide and presents a zoonotic concern. Most poxviruses produce diseases of similar clinical signs in affected animals, which are impossible to differentiate clinically or by serology. It is, therefore, vital to use molecular assays to rapidly identify the causative agents of poxvirus infections. This study aimed to detect, diagnose, and characterize the causative agent of pox-like skin lesions in a cattle herd in Zambia, initially suspected to be infected with Lumpy Skin Disease virus.
Methods: We used a High-Resolution Melting (HRM) analysis assay to detect the PCPV genome and sequenced the major envelope protein (B2L gene) for comparative sequence and phylogenetic analysis.
Results: Our field investigations showed cattle presenting atypical skin lesions and high morbidity within the herd. The laboratory diagnosis, based on the HRM assay revealed PCPV DNA in the samples. Phylogenetic and comparative sequence analyses confirmed PCPV in the samples and revealed genomic differences between samples collected in 2017 and 2018 from the same farm.
Conclusion: Our work is the first documented report of PCPV in Zambia. It shows the strength of molecular methods to diagnose pox-like infections in cattle and discriminate between diseases causing similar clinical signs. This rapid and accurate diagnosis improves the response time for more accurate veterinary interventions.
Databáze: MEDLINE
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